3OWI

Crystal structure of the glycine riboswitch bound to glycine

3OWI の概要

• エントリーDOI: 10.2210/pdb3owi/pdb
• 分子名称: Domain II of glycine riboswitch, GLYCINE, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: gene expression regulator, glycine riboswitch, rna
• 由来する生物種: Vibrio cholerae MJ-1236; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58342.40

構造登録者

Huang, L.,Serganov, A.,Patel, D.J. (登録日: 2010-09-19, 公開日: 2010-12-29, 最終更新日: 2023-09-06)

主引用文献

Huang, L.,Serganov, A.,Patel, D.J.
Structural insights into ligand recognition by a sensing domain of the cooperative glycine riboswitch.
Mol.Cell, 40:774-786, 2010

PubMed Abstract: Glycine riboswitches regulate gene expression by feedback modulation in response to cooperative binding to glycine. Here, we report on crystal structures of the second glycine-sensing domain from the Vibrio cholerae riboswitch in the ligand-bound and unbound states. This domain adopts a three-helical fold that centers on a three-way junction and accommodates glycine within a bulge-containing binding pocket above the junction. Glycine recognition is facilitated by a pair of bound Mg(2+) cations and governed by specific interactions and shape complementarity with the pocket. A conserved adenine extrudes from the binding pocket and intercalates into the junction implying that glycine binding in the context of the complete riboswitch could impact on gene expression by stabilizing the riboswitch junction and regulatory P1 helix. Analysis of riboswitch interactions in the crystal and footprinting experiments indicates that adjacent glycine-sensing modules of the riboswitch could form specific interdomain interactions, thereby potentially contributing to the cooperative response.

PubMed: 21145485
DOI: 10.1016/j.molcel.2010.11.026

実験手法

X-RAY DIFFRACTION (2.845 Å)