3OV5

Atomic structure of the Xanthomonas citri VirB7 globular domain.

3OV5 の概要

• エントリーDOI: 10.2210/pdb3ov5/pdb
• 分子名称: Uncharacterized protein, ISOPROPYL ALCOHOL (3 entities in total)
• 機能のキーワード: type iv secretion system component, virb7 (xac2622), bacterial outer membrane, xanthomonas axonopodis pv citri, protein transport
• 由来する生物種: Xanthomonas axonopodis pv. citri (Citrus canker)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9081.23

構造登録者

Souza, D.P.,Farah, C.S. (登録日: 2010-09-15, 公開日: 2011-06-01, 最終更新日: 2024-04-03)

主引用文献

Souza, D.P.,Andrade, M.O.,Alvarez-Martinez, C.E.,Arantes, G.M.,Farah, C.S.,Salinas, R.K.
A Component of the Xanthomonadaceae Type IV Secretion System Combines a VirB7 Motif with a N0 Domain Found in Outer Membrane Transport Proteins.
Plos Pathog., 7:e1002031-e1002031, 2011

PubMed Abstract: Type IV secretion systems (T4SS) are used by Gram-negative bacteria to translocate protein and DNA substrates across the cell envelope and into target cells. Translocation across the outer membrane is achieved via a ringed tetradecameric outer membrane complex made up of a small VirB7 lipoprotein (normally 30 to 45 residues in the mature form) and the C-terminal domains of the VirB9 and VirB10 subunits. Several species from the genera of Xanthomonas phytopathogens possess an uncharacterized type IV secretion system with some distinguishing features, one of which is an unusually large VirB7 subunit (118 residues in the mature form). Here, we report the NMR and 1.0 Å X-ray structures of the VirB7 subunit from Xanthomonas citri subsp. citri (VirB7(XAC2622)) and its interaction with VirB9. NMR solution studies show that residues 27-41 of the disordered flexible N-terminal region of VirB7(XAC2622) interact specifically with the VirB9 C-terminal domain, resulting in a significant reduction in the conformational freedom of both regions. VirB7(XAC2622) has a unique C-terminal domain whose topology is strikingly similar to that of N0 domains found in proteins from different systems involved in transport across the bacterial outer membrane. We show that VirB7(XAC2622) oligomerizes through interactions involving conserved residues in the N0 domain and residues 42-49 within the flexible N-terminal region and that these homotropic interactions can persist in the presence of heterotropic interactions with VirB9. Finally, we propose that VirB7(XAC2622) oligomerization is compatible with the core complex structure in a manner such that the N0 domains form an extra layer on the perimeter of the tetradecameric ring.

PubMed: 21589901
DOI: 10.1371/journal.ppat.1002031

実験手法

X-RAY DIFFRACTION (1.04 Å)