3OUV

SeMet Derivative of L512M mutant of PASTA domain 3 of Mycobacterium tuberculosis PknB

3OUV の概要

• エントリーDOI: 10.2210/pdb3ouv/pdb
• 分子名称: Serine/threonine protein kinase (2 entities in total)
• 機能のキーワード: protein-ligand interaction, transferase
• 由来する生物種: Mycobacterium tuberculosis H37Ra
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7321.95

構造登録者

Prigozhin, D.M.,Chen, T.Y.,Alber, T. (登録日: 2010-09-15, 公開日: 2011-09-21, 最終更新日: 2024-11-06)

主引用文献

Prigozhin, D.M.,Papavinasasundaram, K.G.,Baer, C.E.,Murphy, K.C.,Moskaleva, A.,Chen, T.Y.,Alber, T.,Sassetti, C.M.
Structural and Genetic Analyses of the Mycobacterium tuberculosis Protein Kinase B Sensor Domain Identify a Potential Ligand-binding Site.
J.Biol.Chem., 291:22961-22969, 2016

PubMed Abstract: Monitoring the environment with serine/threonine protein kinases is critical for growth and survival of Mycobacterium tuberculosis, a devastating human pathogen. Protein kinase B (PknB) is a transmembrane serine/threonine protein kinase that acts as an essential regulator of mycobacterial growth and division. The PknB extracellular domain (ECD) consists of four repeats homologous to penicillin-binding protein and serine/threonine kinase associated (PASTA) domains, and binds fragments of peptidoglycan. These properties suggest that PknB activity is modulated by ECD binding to peptidoglycan substructures, however, the molecular mechanisms underpinning PknB regulation remain unclear. In this study, we report structural and genetic characterization of the PknB ECD. We determined the crystal structures of overlapping ECD fragments at near atomic resolution, built a model of the full ECD, and discovered a region on the C-terminal PASTA domain that has the properties of a ligand-binding site. Hydrophobic interaction between this surface and a bound molecule of citrate was observed in a crystal structure. Our genetic analyses in M. tuberculosis showed that nonfunctional alleles were produced either by deletion of any of single PASTA domain or by mutation of individual conserved residues lining the putative ligand-binding surface of the C-terminal PASTA repeat. These results define two distinct structural features necessary for PknB signal transduction, a fully extended ECD and a conserved, membrane-distal putative ligand-binding site.

PubMed: 27601474
DOI: 10.1074/jbc.M116.731760

実験手法

X-RAY DIFFRACTION (2.004 Å)