3OSM

Structure of the Kinase Associated Domain-1 (KA1) from Kcc4p

Summary for 3OSM

• Entry DOI: 10.2210/pdb3osm/pdb
• Related: 3OSE 3OST
• Descriptor: serine/threonine-protein kinase KCC4, S,R MESO-TARTARIC ACID, GLYCEROL, ... (4 entities in total)
• Functional Keywords: kinase associated-1(ka1) domain, transferase, lipid binding protein, membrane association, kinase
• Biological source: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast)
• Cellular location: Bud neck: P25389
• Total number of polymer chains: 1
• Total formula weight: 14986.74

Authors

Moravcevic, K.,Lemmon, M.A. (deposition date: 2010-09-09, release date: 2010-12-22, Last modification date: 2024-02-21)

Primary citation

Moravcevic, K.,Mendrola, J.M.,Schmitz, K.R.,Wang, Y.H.,Slochower, D.,Janmey, P.A.,Lemmon, M.A.
Kinase Associated-1 Domains Drive MARK/PAR1 Kinases to Membrane Targets by Binding Acidic Phospholipids.
Cell(Cambridge,Mass.), 143:966-977, 2010

PubMed Abstract: Phospholipid-binding modules such as PH, C1, and C2 domains play crucial roles in location-dependent regulation of many protein kinases. Here, we identify the KA1 domain (kinase associated-1 domain), found at the C terminus of yeast septin-associated kinases (Kcc4p, Gin4p, and Hsl1p) and human MARK/PAR1 kinases, as a membrane association domain that binds acidic phospholipids. Membrane localization of isolated KA1 domains depends on phosphatidylserine. Using X-ray crystallography, we identified a structurally conserved binding site for anionic phospholipids in KA1 domains from Kcc4p and MARK1. Mutating this site impairs membrane association of both KA1 domains and intact proteins and reveals the importance of phosphatidylserine for bud neck localization of yeast Kcc4p. Our data suggest that KA1 domains contribute to "coincidence detection," allowing kinases to bind other regulators (such as septins) only at the membrane surface. These findings have important implications for understanding MARK/PAR1 kinases, which are implicated in Alzheimer's disease, cancer, and autism.

PubMed: 21145462
DOI: 10.1016/j.cell.2010.11.028

Experimental method

X-RAY DIFFRACTION (1.7 Å)