3OKP

Crystal structure of Corynebacterium glutamicum PimB' bound to GDP-Man (orthorhombic crystal form)

3OKP の概要

• エントリーDOI: 10.2210/pdb3okp/pdb
• 関連するPDBエントリー: 3oka 3okc
• 分子名称: GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase, GUANOSINE-5'-DIPHOSPHATE-ALPHA-D-MANNOSE (3 entities in total)
• 機能のキーワード: gt-b fold, alpha-mannosyltransferase, gdp-man, transferase
• 由来する生物種: Corynebacterium glutamicum (Brevibacterium flavum)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43508.12

構造登録者

Batt, S.M.,Jabeen, T.,Besra, G.S.,Futterer, K. (登録日: 2010-08-25, 公開日: 2010-09-15, 最終更新日: 2023-09-06)

主引用文献

Batt, S.M.,Jabeen, T.,Mishra, A.K.,Veerapen, N.,Krumbach, K.,Eggeling, L.,Besra, G.S.,Futterer, K.
Acceptor substrate discrimination in phosphatidyl-myo-inositol mannoside synthesis: structural and mutational analysis of mannosyltransferase Corynebacterium glutamicum PimB'.
J.Biol.Chem., 285:37741-37752, 2010

PubMed Abstract: Long term survival of the pathogen Mycobacterium tuberculosis in humans is linked to the immunomodulatory potential of its complex cell wall glycolipids, which include the phosphatidylinositol mannoside (PIM) series as well as the related lipomannan and lipoarabinomannan glycoconjugates. PIM biosynthesis is initiated by a set of cytosolic α-mannosyltransferases, catalyzing glycosyl transfer from the activated saccharide donor GDP-α-D-mannopyranose to the acceptor phosphatidyl-myo-inositol (PI) in an ordered and regio-specific fashion. Herein, we report the crystal structure of mannosyltransferase Corynebacterium glutamicum PimB' in complex with nucleotide to a resolution of 2.0 Å. PimB' attaches mannosyl selectively to the 6-OH of the inositol moiety of PI. Two crystal forms and GDP- versus GDP-α-d-mannopyranose-bound complexes reveal flexibility of the nucleotide conformation as well as of the structural framework of the active site. Structural comparison, docking of the saccharide acceptor, and site-directed mutagenesis pin regio-selectivity to a conserved Asp residue in the N-terminal domain that forces presentation of the correct inositol hydroxyl to the saccharide donor.

PubMed: 20843801
DOI: 10.1074/jbc.M110.165407

実験手法

X-RAY DIFFRACTION (2 Å)