Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

3OK2

Crystal structure of the ANA:RNA decamer without lattice translocation defects

Summary for 3OK2
Entry DOI10.2210/pdb3ok2/pdb
Related3OK4
Descriptor(A6C)(A6C)(A6G)(A6U)(A6A)(A6A)(A6U)(A6G)(A6C)(A6C)(PO4), GGCAUUACGG (3 entities in total)
Functional Keywordsrna, hybrid, ana, sugar modification, altritol sugar rings
Total number of polymer chains6
Total formula weight19713.35
Authors
Ovaere, M.,Van Meervelt, L. (deposition date: 2010-08-24, release date: 2011-11-30, Last modification date: 2024-02-21)
Primary citationOvaere, M.,Sponer, J.,Sponer, J.E.,Herdewijn, P.,Van Meervelt, L.
How does hydroxyl introduction influence the double helical structure: the stabilization of an altritol nucleic acid:ribonucleic acid duplex.
Nucleic Acids Res., 40:7573-7583, 2012
Cited by
PubMed Abstract: Altritol nucleic acids (ANAs) are a promising new tool in the development of artificial small interfering ribonucleic acids (siRNAs) for therapeutical applications. To mimic the siRNA:messenger RNA (mRNA) interactions, the crystal structure of the ANA:RNA construct a(CCGUAAUGCC-P):r(GGCAUUACGG) was determined to 1.96 Å resolution which revealed the hybrid to form an A-type helix. As this A-form is a major requirement in the RNAi process, this crystal structure confirms the potential of altritol-modified siRNAs. Moreover, in the ANA strands, a new type of intrastrand interactions was found between the O2' hydroxyl group of one residue and the sugar ring O4' atom of the next residue. These interactions were further investigated by quantum chemical methods. Besides hydration effects, these intrastrand hydrogen bonds may also contribute to the stability of ANA:RNA duplexes.
PubMed: 22638588
DOI: 10.1093/nar/gks470
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.963 Å)
Structure validation

237992

数据于2025-06-25公开中

PDB statisticsPDBj update infoContact PDBjnumon