3OK2

Crystal structure of the ANA:RNA decamer without lattice translocation defects

3OK2 の概要

• エントリーDOI: 10.2210/pdb3ok2/pdb
• 関連するPDBエントリー: 3OK4
• 分子名称: (A6C)(A6C)(A6G)(A6U)(A6A)(A6A)(A6U)(A6G)(A6C)(A6C)(PO4), GGCAUUACGG (3 entities in total)
• 機能のキーワード: rna, hybrid, ana, sugar modification, altritol sugar rings
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 19713.35

構造登録者

Ovaere, M.,Van Meervelt, L. (登録日: 2010-08-24, 公開日: 2011-11-30, 最終更新日: 2024-02-21)

主引用文献

Ovaere, M.,Sponer, J.,Sponer, J.E.,Herdewijn, P.,Van Meervelt, L.
How does hydroxyl introduction influence the double helical structure: the stabilization of an altritol nucleic acid:ribonucleic acid duplex.
Nucleic Acids Res., 40:7573-7583, 2012

PubMed Abstract: Altritol nucleic acids (ANAs) are a promising new tool in the development of artificial small interfering ribonucleic acids (siRNAs) for therapeutical applications. To mimic the siRNA:messenger RNA (mRNA) interactions, the crystal structure of the ANA:RNA construct a(CCGUAAUGCC-P):r(GGCAUUACGG) was determined to 1.96 Å resolution which revealed the hybrid to form an A-type helix. As this A-form is a major requirement in the RNAi process, this crystal structure confirms the potential of altritol-modified siRNAs. Moreover, in the ANA strands, a new type of intrastrand interactions was found between the O2' hydroxyl group of one residue and the sugar ring O4' atom of the next residue. These interactions were further investigated by quantum chemical methods. Besides hydration effects, these intrastrand hydrogen bonds may also contribute to the stability of ANA:RNA duplexes.

PubMed: 22638588
DOI: 10.1093/nar/gks470

実験手法

X-RAY DIFFRACTION (1.963 Å)