3OJE

Crystal Structure of the Bacillus cereus Enoyl-Acyl Carrier Protein Reductase (Apo form)

3OJE の概要

• エントリーDOI: 10.2210/pdb3oje/pdb
• 関連するPDBエントリー: 3OJF
• 分子名称: Enoyl-[acyl-carrier-protein] reductase (FabL) (NADPH) (2 entities in total)
• 機能のキーワード: enoyl-acp reductase, apo form, rossmann fold, nad binding, oxidoreductase
• 由来する生物種: Bacillus cereus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27772.43

構造登録者

Kim, S.J.,Ha, B.H.,Kim, K.H.,Hong, S.K.,Suh, S.W.,Kim, E.E. (登録日: 2010-08-22, 公開日: 2010-09-08, 最終更新日: 2024-03-20)

主引用文献

Kim, S.J.,Ha, B.H.,Kim, K.H.,Hong, S.K.,Shin, K.J.,Suh, S.W.,Kim, E.E.
Dimeric and tetrameric forms of enoyl-acyl carrier protein reductase from Bacillus cereus
Biochem.Biophys.Res.Commun., 400:517-522, 2010

PubMed Abstract: Enoyl-[acyl carrier protein] reductase (ENR) is an essential enzyme in type II fatty-acid synthesis that catalyzes the last step in each elongation cycle. Thus far FabI, FabL and FabK have been reported to carry out the reaction, with FabI being the most characterized. Some bacteria have more than one ENR, and Bacillus cereus has two (FabI and FabL) reported. Here, we have determined the crystal structures of the later in the apo form and in the ternary complex with NADP(+) and an indole naphthyridinone inhibitor. The two structures are almost identical, except for the three stretches that are disordered in the apo form. The apo form exists as a homo-dimer in both crystal and solution, while the ternary complex forms a homo-tetramer. The three stretches disordered in the apo structure are important in the cofactor and the inhibitor binding as well as in tetramer formation.

PubMed: 20800575
DOI: 10.1016/j.bbrc.2010.08.083

実験手法

X-RAY DIFFRACTION (3.02 Å)