3OHV

Crystal structure of the human Bach2 POZ domain, form II

3OHV の概要

• エントリーDOI: 10.2210/pdb3ohv/pdb
• 関連するPDBエントリー: 3OHU
• 分子名称: Transcription regulator protein BACH2 (2 entities in total)
• 機能のキーワード: btb/poz domain, transcription
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus (By similarity): Q9BYV9
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 85623.05

構造登録者

Rosbrook, G.O.,Stead, M.A.,Carr, S.B.,Wright, S.C. (登録日: 2010-08-18, 公開日: 2011-11-02, 最終更新日: 2024-11-20)

主引用文献

Rosbrook, G.O.,Stead, M.A.,Carr, S.B.,Wright, S.C.
The structure of the Bach2 POZ-domain dimer reveals an intersubunit disulfide bond.
Acta Crystallogr.,Sect.D, 68:26-34, 2012

PubMed Abstract: Bach2 is a transcriptional repressor that is expressed during specific stages of B-cell development and in neuronal cells. It plays a critical role in modulating class-switch recombination during the differentiation of mature B cells to antibody-secreting plasma cells and it is also an important regulator of apoptotic responses to oxidative stress. Bach2 has been implicated both as an oncogene and as a tumour suppressor in human malignancy. The interaction of Bach2 with its target genes is mediated via its basic leucine-zipper region, whereas the N-terminal POZ domain recruits transcriptional co-repressors and class II histone deacetylases. Here, the crystal structure of the human Bach2 POZ domain is reported at 2.1 Å resolution. The Bach2 POZ-domain dimer resembles the POZ-domain dimers of the POZ zinc finger transcription factors and dimerization is independent of an N-terminal region that has previously been implicated in the dimerization of the POZ basic leucine-zipper protein Bach1. The Bach2 POZ domain crystallized in two forms which differed by the presence of an intersubunit disulfide bond. The intersubunit disulfide bond is present both in bacterially expressed Bach2 POZ domain in solution and in protein expressed in transfected eukaryotic cells. These crystal structures will be relevant for understanding the regulation of Bach2 in response to oxidative stress and for the design of therapeutics that target the Bach2 POZ domain in human malignancy.

PubMed: 22194330
DOI: 10.1107/S0907444911048335

実験手法

X-RAY DIFFRACTION (2.2 Å)