3OED

The structure of the complex between complement receptor CR2 and its ligand complement fragment C3d

3OED の概要

• エントリーDOI: 10.2210/pdb3oed/pdb
• 関連するPDBエントリー: 1C3D 1GHQ
• 分子名称: Complement C3, Complement receptor type 2 (3 entities in total)
• 機能のキーワード: complement receptor, complement fragment c3d, b cell, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P01024; Secreted: P20023
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 99153.63

構造登録者

Isenman, D.E.,van den Elsen, J.M.H. (登録日: 2010-08-12, 公開日: 2011-05-11, 最終更新日: 2024-10-16)

主引用文献

van den Elsen, J.M.,Isenman, D.E.
A crystal structure of the complex between human complement receptor 2 and its ligand C3d.
Science, 332:608-611, 2011

PubMed Abstract: The interaction of complement receptor 2 (CR2)--which is present on B cells and follicular dendritic cells--with its antigen-bound ligand C3d results in an enhanced antibody response, thus providing an important link between the innate and adaptive immune systems. Although a cocrystal structure of a complex between C3d and the ligand-binding domains of CR2 has been published, several aspects of this structure, including the position in C3d of the binding interface, remained controversial because of disagreement with biochemical data. We now report a cocrystal structure of a CR2(SCR1-2):C3d complex at 3.2 angstrom resolution in which the interaction interfaces differ markedly from the previously published structure and are consistent with the biochemical data. It is likely that, in the previous structure, the interaction was influenced by the presence of zinc acetate additive in the crystallization buffer, leading to a nonphysiological complex. Detailed knowledge of the binding interface now at hand gives the potential to exploit the interaction in vaccine design or in therapeutics directed against autoreactive B cells.

PubMed: 21527715
DOI: 10.1126/science.1201954

実験手法

X-RAY DIFFRACTION (3.16 Å)