3O3X

Crystal structure of gp41-5, a single-chain 5-helix-bundle based on HIV gp41

3O3X の概要

• エントリーDOI: 10.2210/pdb3o3x/pdb
• 関連するPDBエントリー: 3O3Y 3O3Z 3O40 3O42 3O43
• 分子名称: gp41-5, (4S)-2-METHYL-2,4-PENTANEDIOL, ISOPROPYL ALCOHOL, ... (4 entities in total)
• 機能のキーワード: helix bundle, mimic of hiv gp41, viral protein
• 由来する生物種: artificial gene
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22425.09

構造登録者

Horne, W.S.,Johnson, L.M.,Gellman, S.H. (登録日: 2010-07-26, 公開日: 2011-07-27, 最終更新日: 2023-09-06)

主引用文献

Johnson, L.M.,Horne, W.S.,Gellman, S.H.
Broad Distribution of Energetically Important Contacts across an Extended Protein Interface.
J.Am.Chem.Soc., 133:10038-10041, 2011

PubMed Abstract: Infection of cells by HIV depends upon profound structural rearrangements within the trimeric viral protein gp41. Critical to this process is the formation of a six-helix bundle in which a set of three N-terminal heptad repeat (NHR) helices assemble to form a core displaying long grooves that provide docking sites for three C-terminal heptad repeat (CHR) helices. We report experiments designed to discriminate between two alternative hypotheses regarding the source of affinity between individual CHR helices and the complementary groove: (1) affinity is dominated by interactions of a small cluster of side chains at one end of the CHR helix; or (2) affinity depends upon interactions distributed across the long CHR helix. We have employed two complementary experimental designs, and results from both favor the latter hypothesis.

PubMed: 21644542
DOI: 10.1021/ja203358t

実験手法

X-RAY DIFFRACTION (1.45 Å)