3O31

E81Q mutant of MtNAS in complex with a reaction intermediate

Summary for 3O31

Entry DOI	10.2210/pdb3o31/pdb
Related	3FPE 3FPF 3FPG 3FPH 3FPJ
Descriptor	ThermoNicotianamine Synthase, N-[(3S)-3-amino-3-carboxypropyl]-L-glutamic acid, BROMIDE ION, ... (4 entities in total)
Functional Keywords	rossmann fold, thermonicotianamine synthase, biosynthetic protein
Biological source	Methanothermobacter thermautotrophicus str. Delta H
Total number of polymer chains	2
Total formula weight	68182.80
Authors	Dreyfus, C.,Pignol, D.,Arnoux, P. (deposition date: 2010-07-23, release date: 2011-06-08, Last modification date: 2024-10-09)
Primary citation	Dreyfus, C.,Larrouy, M.,Cavelier, F.,Martinez, J.,Pignol, D.,Arnoux, P. The crystallographic structure of thermoNicotianamine synthase with a synthetic reaction intermediate highlights the sequential processing mechanism. Chem.Commun.(Camb.), 47:5825-5827, 2011 Cited by PubMed Abstract: We determined the three-dimensional structure of a complex between an archaeal nicotianamine synthase homologue and a chemically synthesised reaction intermediate. This structure suggests that the enzymes cavity allows both an ordered substrate binding and provides energetic coupling of the reaction intermediate formation and translocation. PubMed: 21487608 DOI: 10.1039/c1cc10565e PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.7 Å)

Structure validation