3O26

The structure of salutaridine reductase from Papaver somniferum.

3O26 の概要

• エントリーDOI: 10.2210/pdb3o26/pdb
• 分子名称: Salutaridine reductase, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (3 entities in total)
• 機能のキーワード: short chain dehydrogenase/reductases, oxidoreductase
• 由来する生物種: Papaver somniferum (Opium poppy)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34828.05

構造登録者

Higashi, Y.,Kutchen, T.M.,Smith, T.J. (登録日: 2010-07-22, 公開日: 2010-12-15, 最終更新日: 2024-11-20)

主引用文献

Higashi, Y.,Kutchan, T.M.,Smith, T.J.
The atomic structure of salutaridine reductase from the opium poppy Papaver somniferum.
J.Biol.Chem., 66:163-166, 2010

PubMed Abstract: The opium poppy (Papaver somniferum L.) is one of the oldest known medicinal plants. In the biosynthetic pathway for morphine and codeine, salutaridine is reduced to salutaridinol by salutaridine reductase (SalR; EC 1.1.1.248) using NADPH as coenzyme. Here, we report the atomic structure of SalR to a resolution of ∼1.9 Å in the presence of NADPH. The core structure is highly homologous to other members of the short chain dehydrogenase/reductase family. The major difference is that the nicotinamide moiety and the substrate-binding pocket are covered by a loop (residues 265-279), on top of which lies a large "flap"-like domain (residues 105-140). This configuration appears to be a combination of the two common structural themes found in other members of the short chain dehydrogenase/reductase family. Previous modeling studies suggested that substrate inhibition is due to mutually exclusive productive and nonproductive modes of substrate binding in the active site. This model was tested via site-directed mutagenesis, and a number of these mutations abrogated substrate inhibition. However, the atomic structure of SalR shows that these mutated residues are instead distributed over a wide area of the enzyme, and many are not in the active site. To explain how residues distal to the active site might affect catalysis, a model is presented whereby SalR may undergo significant conformational changes during catalytic turnover.

PubMed: 21169353
DOI: 10.1074/jbc.M110.168633

実験手法

X-RAY DIFFRACTION (1.91 Å)