3O1V

Iron-Catalyzed Oxidation Intermediates Captured in A DNA Repair Dioxygenase

3O1V の概要

• エントリーDOI: 10.2210/pdb3o1v/pdb
• 関連するPDBエントリー: 3O1M 3O1O 3O1P 3O1R 3O1S 3O1T 3O1U
• 分子名称: Alpha-ketoglutarate-dependent dioxygenase AlkB, DNA (5'-D(*AP*GP*GP*TP*AP*AP*(MDJ)P*AP*CP*CP*GP*T)-3'), DNA (5'-D(*AP*AP*CP*GP*GP*TP*AP*TP*TP*AP*CP*CP*T)-3'), ... (6 entities in total)
• 機能のキーワード: demethylase, oxidoreductase
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 30846.41

構造登録者

Yi, C.,Jia, G.,Hou, G.,Dai, Q.,Zhang, W.,Zheng, G.,Jian, X.,Yang, C.-G.,Cui, Q.,He, C. (登録日: 2010-07-22, 公開日: 2010-11-17, 最終更新日: 2024-10-30)

主引用文献

Yi, C.,Jia, G.,Hou, G.,Dai, Q.,Zhang, W.,Zheng, G.,Jian, X.,Yang, C.G.,Cui, Q.,He, C.
Iron-catalysed oxidation intermediates captured in a DNA repair dioxygenase.
Nature, 468:330-333, 2010

PubMed Abstract: Mononuclear iron-containing oxygenases conduct a diverse variety of oxidation functions in biology, including the oxidative demethylation of methylated nucleic acids and histones. Escherichia coli AlkB is the first such enzyme that was discovered to repair methylated nucleic acids, which are otherwise cytotoxic and/or mutagenic. AlkB human homologues are known to play pivotal roles in various processes. Here we present structural characterization of oxidation intermediates for these demethylases. Using a chemical cross-linking strategy, complexes of AlkB-double stranded DNA (dsDNA) containing 1,N(6)-etheno adenine (εA), N(3)-methyl thymine (3-meT) and N(3)-methyl cytosine (3-meC) are stabilized and crystallized, respectively. Exposing these crystals, grown under anaerobic conditions containing iron(II) and α-ketoglutarate (αKG), to dioxygen initiates oxidation in crystallo. Glycol (from εA) and hemiaminal (from 3-meT) intermediates are captured; a zwitterionic intermediate (from 3-meC) is also proposed, based on crystallographic observations and computational analysis. The observation of these unprecedented intermediates provides direct support for the oxidative demethylation mechanism for these demethylases. This study also depicts a general mechanistic view of how a methyl group is oxidatively removed from different biological substrates.

PubMed: 21068844
DOI: 10.1038/nature09497

実験手法

X-RAY DIFFRACTION (1.9 Å)