3O0R

Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment

3O0R の概要

• エントリーDOI: 10.2210/pdb3o0r/pdb
• 分子名称: antibody fab fragment light chain, antibody fab fragment heavy chain, Nitric oxide reductase subunit B, ... (10 entities in total)
• 機能のキーワード: oxidoreductase, electron transport, heme, iron, membrane, cytoplasmic membrane, immune system-oxidoreductase complex, immune system/oxidoreductase
• 由来する生物種: Mus musculus; 詳細
• 細胞内の位置: Cell membrane; Multi-pass membrane protein (Potential): Q59647; Cell membrane; Single-pass membrane protein: Q59646
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 118379.16

構造登録者

Hino, T.,Matsumoto, Y.,Nagano, S.,Sugimoto, H.,Fukumori, Y.,Murata, T.,Iwata, S.,Shiro, Y. (登録日: 2010-07-20, 公開日: 2010-12-29, 最終更新日: 2024-10-30)

主引用文献

Hino, T.,Matsumoto, Y.,Nagano, S.,Sugimoto, H.,Fukumori, Y.,Murata, T.,Iwata, S.,Shiro, Y.
Structural basis of biological N2O generation by bacterial nitric oxide reductase
Science, 330:1666-1670, 2010

PubMed Abstract: Nitric oxide reductase (NOR) is an iron-containing enzyme that catalyzes the reduction of nitric oxide (NO) to generate a major greenhouse gas, nitrous oxide (N(2)O). Here, we report the crystal structure of NOR from Pseudomonas aeruginosa at 2.7 angstrom resolution. The structure reveals details of the catalytic binuclear center. The non-heme iron (Fe(B)) is coordinated by three His and one Glu ligands, but a His-Tyr covalent linkage common in cytochrome oxidases (COX) is absent. This structural characteristic is crucial for NOR reaction. Although the overall structure of NOR is closely related to COX, neither the D- nor K-proton pathway, which connect the COX active center to the intracellular space, was observed. Protons required for the NOR reaction are probably provided from the extracellular side.

PubMed: 21109633
DOI: 10.1126/science.1195591

実験手法

X-RAY DIFFRACTION (2.7 Å)