3NZM

Crystal structure of DNAE intein with N-extein in redox trap

Summary for 3NZM

Entry DOI	10.2210/pdb3nzm/pdb
Related	1ZD7 1ZDE
Descriptor	DNA polymerase III subunit alpha, SULFATE ION (3 entities in total)
Functional Keywords	disulfide bond, transferase
Biological source	Synechocystis sp. PCC 6803
Cellular location	Cytoplasm (By similarity): P74750
Total number of polymer chains	1
Total formula weight	18976.35
Authors	Van Roey, P.,Belfort, M.,Callahan, B.P. (deposition date: 2010-07-16, release date: 2011-06-01, Last modification date: 2023-11-15)
Primary citation	Callahan, B.P.,Topilina, N.I.,Stanger, M.J.,Van Roey, P.,Belfort, M. Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications. Nat.Struct.Mol.Biol., 18:630-633, 2011 Cited by PubMed Abstract: Here we describe self-splicing proteins, called inteins, that function as redox-responsive switches in bacteria. Redox regulation was achieved by engineering a disulfide bond between the intein's catalytic cysteine and a cysteine in the flanking 'extein' sequence. This interaction was validated by an X-ray structure, which includes a transient splice junction. A natural analog of the designed system was identified in Pyrococcus abyssi, suggesting an unprecedented form of adaptive, post-translational regulation. PubMed: 21460844 DOI: 10.1038/nsmb.2041 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.55 Å)

Structure validation