3NTY

Crystal structure of AKR1C1 in complex with NADP and 5-Phenyl,3-chlorosalicylic acid

3NTY の概要

• エントリーDOI: 10.2210/pdb3nty/pdb
• 分子名称: Aldo-keto reductase family 1 member C1, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, 5-chloro-4-hydroxybiphenyl-3-carboxylic acid, ... (5 entities in total)
• 機能のキーワード: aldo-keto reductase, 20 alpha hydroxysteroid dehydrogenase, akr1c1, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: Q04828
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37896.81

構造登録者

Dhagat, U.,El-Kabbani, O. (登録日: 2010-07-06, 公開日: 2011-04-13, 最終更新日: 2023-11-01)

主引用文献

El-Kabbani, O.,Dhagat, U.,Soda, M.,Endo, S.,Matsunaga, T.,Hara, A.
Probing the inhibitor selectivity pocket of human 20 alpha-hydroxysteroid dehydrogenase (AKR1C1) with X-ray crystallography and site-directed mutagenesis
Bioorg.Med.Chem.Lett., 21:2564-2567, 2011

PubMed Abstract: Human 20α-hydroxysteroid dehydrogenase (AKR1C1) is an important drug target due to its role in the development of lung and endometrial cancers, premature birth and neuronal disorders. We report the crystal structure of AKR1C1 complexed with the first structure-based designed inhibitor 3-chloro-5-phenylsalicylic acid (K(i)=0.86 nM) bound in the active site. The binding of 3-chloro-5-phenylsalicylic acid to AKR1C1 resulted in a conformational change in the side chain of Phe311 to accommodate the bulky phenyl ring substituent at the 5-position of the inhibitor. The contributions of the nonconserved residues Leu54, Leu306, Leu308 and Phe311 to the binding were further investigated by site-directed mutagenesis, and the effects of the mutations on the K(i) value were determined. The Leu54Val and Leu306Ala mutations resulted in 6- and 81-fold increases, respectively, in K(i) values compared to the wild-type enzyme, while the remaining mutations had little or no effects.

PubMed: 21414777
DOI: 10.1016/j.bmcl.2011.01.076

実験手法

X-RAY DIFFRACTION (1.87 Å)