3NTH

Crystal structure of Tudor and Aubergine [R13(me2s)] complex

Summary for 3NTH

• Entry DOI: 10.2210/pdb3nth/pdb
• Related: 3NTI 3NTK
• Descriptor: Maternal protein tudor, peptide from Aubergine (3 entities in total)
• Functional Keywords: tudor domain, ob-fold, germ cell formation, transcription
• Biological source: Drosophila melanogaster (Fruit fly); More
• Total number of polymer chains: 2
• Total formula weight: 20269.84

Authors

Liu, H.P.,Huang, Y.,Li, Z.Z.,Gong, W.M.,Xu, R.M. (deposition date: 2010-07-05, release date: 2010-09-15, Last modification date: 2024-11-13)

Primary citation

Liu, H.P.,Wang, J.Y.,Huang, Y.,Li, Z.Z.,Gong, W.M.,Lehmann, R.,Xu, R.M.
Structural basis for methylarginine-dependent recognition of Aubergine by Tudor
Genes Dev., 24:1876-1881, 2010

PubMed Abstract: Piwi proteins are modified by symmetric dimethylation of arginine (sDMA), and the methylarginine-dependent interaction with Tudor domain proteins is critical for their functions in germline development. Cocrystal structures of an extended Tudor domain (eTud) of Drosophila Tudor with methylated peptides of Aubergine, a Piwi family protein, reveal that sDMA is recognized by an asparagine-gated aromatic cage. Furthermore, the unexpected Tudor-SN/p100 fold of eTud is important for sensing the position of sDMA. The structural information provides mechanistic insights into sDMA-dependent Piwi-Tudor interaction, and the recognition of sDMA by Tudor domains in general.

PubMed: 20713507
DOI: 10.1101/gad.1956010

Experimental method

X-RAY DIFFRACTION (2.8 Å)