3NRP

Crystal structure of 'as isolated' uropathogenic E. coli strain F11 FetP recombinantly expressed in the periplasm of E. coli BL21(DE3)

3NRP の概要

• エントリーDOI: 10.2210/pdb3nrp/pdb
• 関連するPDBエントリー: 3NRQ
• 分子名称: Periplasmic protein-probably involved in high-affinity Fe2+ transport (2 entities in total)
• 機能のキーワード: immunoglobulin-like fold, iron transporter, copper binding, transport protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 70643.78

構造登録者

Chan, A.C.K.,Murphy, M.E.P. (登録日: 2010-06-30, 公開日: 2011-05-18, 最終更新日: 2023-12-27)

主引用文献

Koch, D.,Chan, A.C.,Murphy, M.E.,Lilie, H.,Grass, G.,Nies, D.H.
Characterization of a Dipartite Iron Uptake System from Uropathogenic Escherichia coli Strain F11.
J.Biol.Chem., 286:25317-25330, 2011

PubMed Abstract: In the uropathogenic Escherichia coli strain F11, in silico genome analysis revealed the dicistronic iron uptake operon fetMP, which is under iron-regulated control mediated by the Fur regulator. The expression of fetMP in a mutant strain lacking known iron uptake systems improved growth under iron depletion and increased cellular iron accumulation. FetM is a member of the iron/lead transporter superfamily and is essential for iron uptake by the Fet system. FetP is a periplasmic protein that enhanced iron uptake by FetM. Recombinant FetP bound Cu(II) and the iron analog Mn(II) at distinct sites. The crystal structure of the FetP dimer reveals a copper site in each FetP subunit that adopts two conformations: CuA with a tetrahedral geometry composed of His(44), Met(90), His(97), and His(127), and CuB, a second degenerate octahedral geometry with the addition of Glu(46). The copper ions of each site occupy distinct positions and are separated by ∼1.3 Å. Nearby, a putative additional Cu(I) binding site is proposed as an electron source that may function with CuA/CuB displacement to reduce Fe(III) for transport by FetM. Together, these data indicate that FetMP is an additional iron uptake system composed of a putative iron permease and an iron-scavenging and potentially iron-reducing periplasmic protein.

PubMed: 21596746
DOI: 10.1074/jbc.M111.222745

実験手法

X-RAY DIFFRACTION (1.6 Å)