3NRK

The crystal structure of the leptospiral hypothetical protein LIC12922

3NRK の概要

• エントリーDOI: 10.2210/pdb3nrk/pdb
• 分子名称: LIC12922 (1 entity in total)
• 機能のキーワード: nc domain, parvulin domain, sura homology, probable chaperone, unknown function
• 由来する生物種: Leptospira interrogans serovar Copenhageni
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37840.01

構造登録者

Giuseppe, P.O.,Atzingen, M.V.,Nascimento, A.L.T.O.,Zanchin, N.I.T.,Guimaraes, B.G. (登録日: 2010-06-30, 公開日: 2010-11-24, 最終更新日: 2023-12-27)

主引用文献

Giuseppe, P.O.,Von Atzingen, M.,Nascimento, A.L.,Zanchin, N.I.,Guimaraes, B.G.
The crystal structure of the leptospiral hypothetical protein LIC12922 reveals homology with the periplasmic chaperone SurA.
J.Struct.Biol., 173:312-322, 2011

PubMed Abstract: Leptospirosis is a world spread zoonosis caused by members of the genus Leptospira. Although leptospires were identified as the causal agent of leptospirosis almost 100 years ago, little is known about their biology, which hinders the development of new treatment and prevention strategies. One of the several aspects of the leptospiral biology not yet elucidated is the process by which outer membrane proteins (OMPs) traverse the periplasm and are inserted into the outer membrane. The crystal structure determination of the conserved hypothetical protein LIC12922 from Leptospira interrogans revealed a two domain protein homologous to the Escherichia coli periplasmic chaperone SurA. The LIC12922 NC-domain is structurally related to the chaperone modules of E. coli SurA and trigger factor, whereas the parvulin domain is devoid of peptidyl prolyl cis-trans isomerase activity. Phylogenetic analyses suggest a relationship between LIC12922 and the chaperones PrsA, PpiD and SurA. Based on our structural and evolutionary analyses, we postulate that LIC12922 is a periplasmic chaperone involved in OMPs biogenesis in Leptospira spp. Since LIC12922 homologs were identified in all spirochetal genomes sequenced to date, this assumption may have implications for the OMPs biogenesis studies not only in leptospires but in the entire Phylum Spirochaetes.

PubMed: 20970503
DOI: 10.1016/j.jsb.2010.10.009

実験手法

X-RAY DIFFRACTION (3.1 Å)