3NPW

In silico designed of an improved Kemp eliminase KE70 mutant by computational design and directed evolution

3NPW の概要

• エントリーDOI: 10.2210/pdb3npw/pdb
• 関連するPDBエントリー: 3NPU 3NPV 3NPX 3NQ2 3NQ8 3NQV 3NR0
• 分子名称: deoxyribose phosphate aldolase (2 entities in total)
• 機能のキーワード: tim, structural genomics, israel structural proteomics center, ispc, lyase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 56025.91

構造登録者

Khersonsky, O.,Rothlisberge, D.,Wollacott, A.M.,Dym, O.,Baker, D.,Tawfik, D.S.,Israel Structural Proteomics Center (ISPC) (登録日: 2010-06-29, 公開日: 2011-02-09, 最終更新日: 2023-11-01)

• 主引用文献: Khersonsky, O.,Rothlisberger, D.,Wollacott, A.M.,Murphy, P.,Dym, O.,Albeck, S.,Kiss, G.,Houk, K.N.,Baker, D.,Tawfik, D.S.; Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution; J.Mol.Biol., 407:391-412, 2011; PubMed: 21277311; DOI: 10.1016/j.jmb.2011.01.041

実験手法

X-RAY DIFFRACTION (2.14 Å)