3NPE

Structure of VP14 in complex with oxygen

3NPE の概要

• エントリーDOI: 10.2210/pdb3npe/pdb
• 分子名称: 9-cis-epoxycarotenoid dioxygenase 1, chloroplastic, FE (II) ION, OXYGEN MOLECULE, ... (6 entities in total)
• 機能のキーワード: dioxygenase, seven blade beta propeller, abscisic acid, non heme iron, oxidoreductase
• 由来する生物種: Zea mays (maize)
• 細胞内の位置: Plastid, chloroplast stroma : O24592
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58016.01

構造登録者

Messing, S.A.,Gabelli, S.B.,Amzel, L.M. (登録日: 2010-06-28, 公開日: 2010-11-10, 最終更新日: 2024-11-20)

主引用文献

Messing, S.A.,Gabelli, S.B.,Echeverria, I.,Vogel, J.T.,Guan, J.C.,Tan, B.C.,Klee, H.J.,McCarty, D.R.,Amzel, L.M.
Structural insights into maize viviparous14, a key enzyme in the biosynthesis of the phytohormone abscisic acid.
Plant Cell, 22:2970-2980, 2010

PubMed Abstract: The key regulatory step in the biosynthesis of abscisic acid (ABA), a hormone central to the regulation of several important processes in plants, is the oxidative cleavage of the 11,12 double bond of a 9-cis-epoxycarotenoid. The enzyme viviparous14 (VP14) performs this cleavage in maize (Zea mays), making it a target for the rational design of novel chemical agents and genetic modifications that improve plant behavior through the modulation of ABA levels. The structure of VP14, determined to 3.2-Å resolution, provides both insight into the determinants of regio- and stereospecificity of this enzyme and suggests a possible mechanism for oxidative cleavage. Furthermore, mutagenesis of the distantly related CCD1 of maize shows how the VP14 structure represents a template for all plant carotenoid cleavage dioxygenases (CCDs). In addition, the structure suggests how VP14 associates with the membrane as a way of gaining access to its membrane soluble substrate.

PubMed: 20884803
DOI: 10.1105/tpc.110.074815

実験手法

X-RAY DIFFRACTION (3.2 Å)