3NM9

HMGD(M13A)-DNA complex

3NM9 の概要

• エントリーDOI: 10.2210/pdb3nm9/pdb
• 関連するPDBエントリー: 1qrv
• 分子名称: High mobility group protein D, DNA 5'-D(*G*GP*CP*GP*AP*TP*AP*TP*CP*GP*C)-3' (3 entities in total)
• 機能のキーワード: high mobility group, dna bending, non-sequence-specific, hmg domain, chromosomal protein, dna, gene regulation-dna complex, gene regulation/dna
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• 細胞内の位置: Nucleus: Q05783
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 83604.48

構造登録者

Churchill, M.E.A.,Klass, J.,Zoetewey, D.L. (登録日: 2010-06-22, 公開日: 2010-09-22, 最終更新日: 2023-09-06)

主引用文献

Churchill, M.E.,Klass, J.,Zoetewey, D.L.
Structural analysis of HMGD-DNA complexes reveals influence of intercalation on sequence selectivity and DNA bending.
J.Mol.Biol., 403:88-102, 2010

PubMed Abstract: The ubiquitous, eukaryotic, high-mobility group box (HMGB) chromosomal proteins promote many chromatin-mediated cellular activities through their non-sequence-specific binding and bending of DNA. Minor-groove DNA binding by the HMG box results in substantial DNA bending toward the major groove owing to electrostatic interactions, shape complementarity, and DNA intercalation that occurs at two sites. Here, the structures of the complexes formed with DNA by a partially DNA intercalation-deficient mutant of Drosophila melanogaster HMGD have been determined by X-ray crystallography at a resolution of 2.85 Å. The six proteins and 50 bp of DNA in the crystal structure revealed a variety of bound conformations. All of the proteins bound in the minor groove, bridging DNA molecules, presumably because these DNA regions are easily deformed. The loss of the primary site of DNA intercalation decreased overall DNA bending and shape complementarity. However, DNA bending at the secondary site of intercalation was retained and most protein-DNA contacts were preserved. The mode of binding resembles the HMGB1 box A-cisplatin-DNA complex, which also lacks a primary intercalating residue. This study provides new insights into the binding mechanisms used by HMG boxes to recognize varied DNA structures and sequences as well as modulate DNA structure and DNA bending.

PubMed: 20800069
DOI: 10.1016/j.jmb.2010.08.031

実験手法

X-RAY DIFFRACTION (2.85 Å)