3NKD

Structure of CRISP-associated protein Cas1 from Escherichia coli str. K-12

3NKD の概要

• エントリーDOI: 10.2210/pdb3nkd/pdb
• 関連するPDBエントリー: 3GOD 3NKE
• 分子名称: CRISPR-associated protein Cas1 (2 entities in total)
• 機能のキーワード: crispr, cas1, ygbt, nuclease, dna recombination, dna repair, immune system
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66470.84

構造登録者

Nocek, B.,Skarina, T.,Beloglazova, N.,Savchenko, A.,Joachimiak, A.,Yakunin, A. (登録日: 2010-06-18, 公開日: 2010-08-25, 最終更新日: 2024-11-20)

主引用文献

Babu, M.,Beloglazova, N.,Flick, R.,Graham, C.,Skarina, T.,Nocek, B.,Gagarinova, A.,Pogoutse, O.,Brown, G.,Binkowski, A.,Phanse, S.,Joachimiak, A.,Koonin, E.V.,Savchenko, A.,Emili, A.,Greenblatt, J.,Edwards, A.M.,Yakunin, A.F.
A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair.
Mol.Microbiol., 79:484-502, 2011

PubMed Abstract: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) and the associated proteins (Cas) comprise a system of adaptive immunity against viruses and plasmids in prokaryotes. Cas1 is a CRISPR-associated protein that is common to all CRISPR-containing prokaryotes but its function remains obscure. Here we show that the purified Cas1 protein of Escherichia coli (YgbT) exhibits nuclease activity against single-stranded and branched DNAs including Holliday junctions, replication forks and 5'-flaps. The crystal structure of YgbT and site-directed mutagenesis have revealed the potential active site. Genome-wide screens show that YgbT physically and genetically interacts with key components of DNA repair systems, including recB, recC and ruvB. Consistent with these findings, the ygbT deletion strain showed increased sensitivity to DNA damage and impaired chromosomal segregation. Similar phenotypes were observed in strains with deletion of CRISPR clusters, suggesting that the function of YgbT in repair involves interaction with the CRISPRs. These results show that YgbT belongs to a novel, structurally distinct family of nucleases acting on branched DNAs and suggest that, in addition to antiviral immunity, at least some components of the CRISPR-Cas system have a function in DNA repair.

PubMed: 21219465
DOI: 10.1111/j.1365-2958.2010.07465.x

実験手法

X-RAY DIFFRACTION (1.95 Å)