3NJO

X-ray crystal structure of the Pyr1-pyrabactin A complex

3NJO の概要

• エントリーDOI: 10.2210/pdb3njo/pdb
• 関連するPDBエントリー: 3NJ0 3NJ1
• 分子名称: Abscisic acid receptor PYR1, 4-bromo-N-(pyridin-2-ylmethyl)naphthalene-1-sulfonamide, SULFATE ION, ... (7 entities in total)
• 機能のキーワード: start, aba, pyr/pyl/rcar, plant hormone, structural genomics, protein structure initiative, psi, center for eukaryotic structural genomics, cesg, hormone receptor
• 由来する生物種: Arabidopsis thaliana (thale-cress)
• 細胞内の位置: Cytoplasm : O49686
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 66929.90

構造登録者

Burgie, E.S.,Bingman, C.A.,Phillips Jr., G.N.,Peterson, F.C.,Volkman, B.F.,Cutler, S.R.,Jensen, D.R.,Center for Eukaryotic Structural Genomics (CESG) (登録日: 2010-06-17, 公開日: 2010-08-18, 最終更新日: 2023-09-06)

主引用文献

Peterson, F.C.,Burgie, E.S.,Park, S.Y.,Jensen, D.R.,Weiner, J.J.,Bingman, C.A.,Chang, C.E.,Cutler, S.R.,Phillips, G.N.,Volkman, B.F.
Structural basis for selective activation of ABA receptors.
Nat.Struct.Mol.Biol., 17:1109-1113, 2010

PubMed Abstract: Changing environmental conditions and lessening fresh water supplies have sparked intense interest in understanding and manipulating abscisic acid (ABA) signaling, which controls adaptive responses to drought and other abiotic stressors. We recently discovered a selective ABA agonist, pyrabactin, and used it to discover its primary target PYR1, the founding member of the PYR/PYL family of soluble ABA receptors. To understand pyrabactin's selectivity, we have taken a combined structural, chemical and genetic approach. We show that subtle differences between receptor binding pockets control ligand orientation between productive and nonproductive modes. Nonproductive binding occurs without gate closure and prevents receptor activation. Observations in solution show that these orientations are in rapid equilibrium that can be shifted by mutations to control maximal agonist activity. Our results provide a robust framework for the design of new agonists and reveal a new mechanism for agonist selectivity.

PubMed: 20729860
DOI: 10.1038/nsmb.1898

実験手法

X-RAY DIFFRACTION (2.473 Å)