3NE1

Mycobacterium tuberculosis Acyl Carrier Protein Synthase in complex with sulfate ion

3NE1 の概要

• エントリーDOI: 10.2210/pdb3ne1/pdb
• 関連するPDBエントリー: 3NE3 3NE9
• 分子名称: Holo-[acyl-carrier-protein] synthase, SULFATE ION (3 entities in total)
• 機能のキーワード: acyl carrier protein synthase, acyl carrier protein, fatty acid synthase, acps, mycobacterium tuberculosis structural proteomics project, transferase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 42443.52

構造登録者

Gokulan, K. (登録日: 2010-06-08, 公開日: 2010-07-21, 最終更新日: 2024-02-21)

主引用文献

Gokulan, K.,Aggarwal, A.,Shipman, L.,Besra, G.S.,Sacchettini, J.C.
Mycobacterium tuberculosis acyl carrier protein synthase adopts two different pH-dependent structural conformations.
Acta Crystallogr.,Sect.D, 67:657-669, 2011

PubMed Abstract: The crystal structures of acyl carrier protein synthase (AcpS) from Mycobacterium tuberculosis (Mtb) and Corynebacterium ammoniagenes determined at pH 5.3 and pH 6.5, respectively, are reported. Comparison of the Mtb apo-AcpS structure with the recently reported structure of the Mtb AcpS-ADP complex revealed that AcpS adopts two different conformations: the orthorhombic and trigonal space-group structures show structural differences in the α2 helix and in the conformation of the α3-α4 connecting loop, which is in a closed conformation. The apo-AcpS structure shows electron density for the entire model and was obtained at lower pH values (4.4-6.0). In contrast, at a higher pH value (6.5) AcpS undergoes significant conformational changes, resulting in disordered regions that show no electron density in the AcpS model. The solved structures also reveal that C. ammoniagenes AcpS undergoes structural rearrangement in two regions, similar to the recently reported Mtb AcpS-ADP complex structure. In vitro reconstitution experiments show that AcpS has a higher post-translational modification activity between pH 4.4 and 6.0 than at pH values above 6.5, where the activity drops owing to the change in conformation. The results show that apo-AcpS and AcpS-ADP adopt different conformations depending upon the pH conditions of the crystallization solution.

PubMed: 21697604
DOI: 10.1107/S0907444911020221

実験手法

X-RAY DIFFRACTION (2.51 Å)