3N2S

Structure of NfrA1 nitroreductase from B. subtilis

3N2S の概要

• エントリーDOI: 10.2210/pdb3n2s/pdb
• 分子名称: NADPH-dependent nitro/flavin reductase, FLAVIN MONONUCLEOTIDE, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: alpga-beta-alpha sandwich, oxidoreductase
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 116628.78

構造登録者

Morera, S.,Gueguen-Chaignon, V.,Meyer, P.,Cortial, S.,Ouazzani, J. (登録日: 2010-05-19, 公開日: 2010-09-15, 最終更新日: 2024-11-06)

主引用文献

Cortial, S.,Chaignon, P.,Iorga, B.I.,Aymerich, S.,Truan, G.,Gueguen-Chaignon, V.,Meyer, P.,Morera, S.,Ouazzani, J.
NADH oxidase activity of Bacillus subtilis nitroreductase NfrA1: insight into its biological role.
Febs Lett., 584:3916-3922, 2010

PubMed Abstract: NfrA1 nitroreductase from the Gram-positive bacterium Bacillus subtilis is a member of the NAD(P)H/FMN oxidoreductase family. Here, we investigated the reactivity, the structure and kinetics of NfrA1, which could provide insight into the unclear biological role of this enzyme. We could show that NfrA1 possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD(+) degrading activity leading to free nicotinamide. Finally, we showed that NfrA1 is able to rapidly scavenge H(2)O(2) produced during the oxidative process or added exogenously.

PubMed: 20727352
DOI: 10.1016/j.febslet.2010.08.019

実験手法

X-RAY DIFFRACTION (1.95 Å)