3N1H

Crystal Structure of StWhy2

3N1H の概要

• エントリーDOI: 10.2210/pdb3n1h/pdb
• 関連するPDBエントリー: 3N1I 3N1J 3N1K 3N1L
• 分子名称: StWhy2, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: stwhy2, single-stranded dna binding protein, plant, potato, whirly, dna binding protein
• 由来する生物種: Solanum tuberosum (Potato)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20190.66

構造登録者

Cappadocia, L.,Brisson, N.,Sygusch, J. (登録日: 2010-05-15, 公開日: 2010-08-11, 最終更新日: 2023-09-06)

主引用文献

Cappadocia, L.,Marechal, A.,Parent, J.S.,Lepage, E.,Sygusch, J.,Brisson, N.
Crystal Structures of DNA-Whirly Complexes and Their Role in Arabidopsis Organelle Genome Repair.
Plant Cell, 22:1849-1867, 2010

PubMed Abstract: DNA double-strand breaks are highly detrimental to all organisms and need to be quickly and accurately repaired. Although several proteins are known to maintain plastid and mitochondrial genome stability in plants, little is known about the mechanisms of DNA repair in these organelles and the roles of specific proteins. Here, using ciprofloxacin as a DNA damaging agent specific to the organelles, we show that plastids and mitochondria can repair DNA double-strand breaks through an error-prone pathway similar to the microhomology-mediated break-induced replication observed in humans, yeast, and bacteria. This pathway is negatively regulated by the single-stranded DNA (ssDNA) binding proteins from the Whirly family, thus indicating that these proteins could contribute to the accurate repair of plant organelle genomes. To understand the role of Whirly proteins in this process, we solved the crystal structures of several Whirly-DNA complexes. These reveal a nonsequence-specific ssDNA binding mechanism in which DNA is stabilized between domains of adjacent subunits and rendered unavailable for duplex formation and/or protein interactions. Our results suggest a model in which the binding of Whirly proteins to ssDNA would favor accurate repair of DNA double-strand breaks over an error-prone microhomology-mediated break-induced replication repair pathway.

PubMed: 20551348
DOI: 10.1105/tpc.109.071399

実験手法

X-RAY DIFFRACTION (2.201 Å)