3N0R

Structure of the PhyR stress response regulator at 1.25 Angstrom resolution

3N0R の概要

• エントリーDOI: 10.2210/pdb3n0r/pdb
• 分子名称: Response regulator, GLYCEROL (3 entities in total)
• 機能のキーワード: sigma factor, receiver, response regulator, two-component signal transduction, signaling protein
• 由来する生物種: Caulobacter vibrioides
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31322.94

構造登録者

Herrou, J.,Crosson, S. (登録日: 2010-05-14, 公開日: 2010-08-11, 最終更新日: 2024-11-20)

主引用文献

Herrou, J.,Foreman, R.,Fiebig, A.,Crosson, S.
A structural model of anti-anti-sigma inhibition by a two-component receiver domain: the PhyR stress response regulator
Mol.Microbiol., 78:290-304, 2010

PubMed Abstract: PhyR is a hybrid stress regulator conserved in α-proteobacteria that contains an N-terminal σ-like (SL) domain and a C-terminal receiver domain. Phosphorylation of the receiver domain is known to promote binding of the SL domain to an anti-σ factor. PhyR thus functions as an anti-anti-σ factor in its phosphorylated state. We present genetic evidence that Caulobacter crescentus PhyR is a phosphorylation-dependent stress regulator that functions in the same pathway as σ(T) and its anti-σ factor, NepR. Additionally, we report the X-ray crystal structure of PhyR at 1.25 Å resolution, which provides insight into the mechanism of anti-anti-σ regulation. Direct intramolecular contact between the PhyR receiver and SL domains spans regions σ₂ and σ₄, likely serving to stabilize the SL domain in a closed conformation. The molecular surface of the receiver domain contacting the SL domain is the structural equivalent of α4-β5-α5, which is known to undergo dynamic conformational change upon phosphorylation in a diverse range of receiver proteins. We propose a structural model of PhyR regulation in which receiver phosphorylation destabilizes the intramolecular interaction between SL and receiver domains, thereby permitting regions σ₂ and σ₄ in the SL domain to open about a flexible connector loop and bind anti-σ factor.

PubMed: 20735776
DOI: 10.1111/j.1365-2958.2010.07323.x

実験手法

X-RAY DIFFRACTION (1.251 Å)