3N0F

Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens)

3N0F の概要

• エントリーDOI: 10.2210/pdb3n0f/pdb
• 関連するPDBエントリー: 3N0G
• 分子名称: Isoprene synthase (2 entities in total)
• 機能のキーワード: terpene cyclase fold, hemiterpene synthase, ddxxd motif, nse/dte motif, lyase
• 由来する生物種: Populus tremula x Populus alba (gray poplar)
• 細胞内の位置: Plastid, chloroplast (Potential): Q9AR86
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 128236.02

構造登録者

Koksal, M.,Christianson, D.W. (登録日: 2010-05-13, 公開日: 2010-07-21, 最終更新日: 2023-09-06)

主引用文献

Koksal, M.,Zimmer, I.,Schnitzler, J.P.,Christianson, D.W.
Structure of isoprene synthase illuminates the chemical mechanism of teragram atmospheric carbon emission.
J.Mol.Biol., 402:363-373, 2010

PubMed Abstract: The X-ray crystal structure of recombinant PcISPS (isoprene synthase from gray poplar hybrid Populus×canescens) has been determined at 2.7 Å resolution, and the structure of its complex with three Mg(2+) and the unreactive substrate analogue dimethylallyl-S-thiolodiphosphate has been determined at 2.8 Å resolution. Analysis of these structures suggests that the generation of isoprene from substrate dimethylallyl diphosphate occurs via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base. This chemical mechanism is responsible for the annual atmospheric emission of 100 Tg of isoprene by terrestrial plant life. Importantly, the PcISPS structure promises to guide future protein engineering studies, potentially leading to hydrocarbon fuels and products that do not rely on traditional petrochemical sources.

PubMed: 20624401
DOI: 10.1016/j.jmb.2010.07.009

実験手法

X-RAY DIFFRACTION (2.7 Å)