3MW4

Crystal structure of beta-neurexin 3 without the splice insert 4

3MW4 の概要

• エントリーDOI: 10.2210/pdb3mw4/pdb
• 関連するPDBエントリー: 3MW2 3MW3
• 分子名称: Neurexin-2-beta, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-D-mannopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: neurexin, lns domain, calcium-binding, cell adhesion, glycoprotein
• 由来する生物種: Mus musculus (mouse)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 60749.93

構造登録者

Jin, X.,Shapiro, L. (登録日: 2010-05-05, 公開日: 2010-07-28, 最終更新日: 2024-11-27)

主引用文献

Koehnke, J.,Katsamba, P.S.,Ahlsen, G.,Bahna, F.,Vendome, J.,Honig, B.,Shapiro, L.,Jin, X.
Splice Form Dependence of beta-Neurexin/Neuroligin Binding Interactions.
Neuron, 67:61-74, 2010

PubMed Abstract: Alternatively spliced beta-neurexins (beta-NRXs) and neuroligins (NLs) are thought to have distinct extracellular binding affinities, potentially providing a beta-NRX/NL synaptic recognition code. We utilized surface plasmon resonance to measure binding affinities between all combinations of alternatively spliced beta-NRX 1-3 and NL 1-3 ectodomains. Binding was observed for all beta-NRX/NL pairs. The presence of the NL1 B splice insertion lowers beta-NRX binding affinity by approximately 2-fold, while beta-NRX splice insertion 4 has small effects that do not synergize with NL splicing. New structures of glycosylated beta-NRXs 1 and 2 containing splice insertion 4 reveal that the insertion forms a new beta strand that replaces the beta10 strand, leaving the NL binding site intact. This helps to explain the limited effect of splice insert 4 on NRX/NL binding affinities. These results provide new structural insights and quantitative binding information to help determine whether and how splice isoform choice plays a role in beta-NRX/NL-mediated synaptic recognition.

PubMed: 20624592
DOI: 10.1016/j.neuron.2010.06.001

実験手法

X-RAY DIFFRACTION (2 Å)