3MPS

Peroxide Bound Oxidized Rubrerythrin from Pyrococcus furiosus

3MPS の概要

• エントリーDOI: 10.2210/pdb3mps/pdb
• 関連するPDBエントリー: 3PWF 3PZA 3QVD
• 分子名称: Rubrerythrin, FE (III) ION, MU-OXO-DIIRON, ... (5 entities in total)
• 機能のキーワード: diiron, rubrerythrin, peroxidase, peroxide, oxidized, oxidoreductase
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 156625.01

構造登録者

Dillard, B.D.,Adams, M.W.W.,Lanzilotta, W.N. (登録日: 2010-04-27, 公開日: 2011-06-22, 最終更新日: 2024-02-21)

主引用文献

Dillard, B.D.,Demick, J.M.,Adams, M.W.,Lanzilotta, W.N.
A cryo-crystallographic time course for peroxide reduction by rubrerythrin from Pyrococcus furiosus.
J.Biol.Inorg.Chem., 16:949-959, 2011

PubMed Abstract: High-resolution crystal structures of Pyrococcus furiosus rubrerythrin (PfRbr) in the resting (all-ferrous) state and at time points following exposure of the crystals to hydrogen peroxide are reported. This approach was possible because of the relativity slow turnover of PfRbr at room temperature. To this end, we were able to perform time-dependent peroxide treatment of the fully reduced enzyme, under strictly anaerobic conditions, in the crystalline state. In this work we demonstrate, for the first time, that turnover of a thermophilic rubrerythrin results in approximately 2-Å movement of one iron atom in the diiron site from a histidine to a carboxylate ligand. These results confirm that, despite the domain-swapped architecture, the hyperthermophilic rubrerythrins also utilize the classic combination of iron sites together with redox-dependent iron toggling to selectively reduce hydrogen peroxide over dioxygen. In addition, we have identified previously unobserved intermediates in the reaction cycle and observed structural changes that may explain the enzyme precipitation observed for the all-iron form of PfRbr upon oxidation to the all-ferric state.

PubMed: 21647777
DOI: 10.1007/s00775-011-0795-6

実験手法

X-RAY DIFFRACTION (2 Å)