3MPP

Botulinum Neurotoxin Type G Receptor Binding Domain

3MPP の概要

• エントリーDOI: 10.2210/pdb3mpp/pdb
• 分子名称: Botulinum neurotoxin type G (2 entities in total)
• 機能のキーワード: beta barrel beta trefoil, toxin
• 由来する生物種: Clostridium botulinum
• 細胞内の位置: Secreted (By similarity): Q60393
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50678.64

構造登録者

Schmitt, J.M.,Lacy, D.B. (登録日: 2010-04-27, 公開日: 2010-06-16, 最終更新日: 2023-09-06)

主引用文献

Schmitt, J.,Karalewitz, A.,Benefield, D.A.,Mushrush, D.J.,Pruitt, R.N.,Spiller, B.W.,Barbieri, J.T.,Lacy, D.B.
Structural analysis of botulinum neurotoxin type G receptor binding .
Biochemistry, 49:5200-5205, 2010

PubMed Abstract: Botulinum neurotoxin (BoNT) binds peripheral neurons at the neuromuscular junction through a dual-receptor mechanism that includes interactions with ganglioside and protein receptors. The receptor identities vary depending on BoNT serotype (A-G). BoNT/B and BoNT/G bind the luminal domains of synaptotagmin I and II, homologous synaptic vesicle proteins. We observe conditions under which BoNT/B binds both Syt isoforms, but BoNT/G binds only SytI. Both serotypes bind ganglioside G(T1b). The BoNT/G receptor-binding domain crystal structure provides a context for examining these binding interactions and a platform for understanding the physiological relevance of different Syt receptor isoforms in vivo.

PubMed: 20507178
DOI: 10.1021/bi100412v

実験手法

X-RAY DIFFRACTION (1.98 Å)