3MMP

Structure of the Qb replicase, an RNA-dependent RNA polymerase consisting of viral and host proteins

3MMP の概要

• エントリーDOI: 10.2210/pdb3mmp/pdb
• 分子名称: Elongation factor Tu 2, Elongation factor Ts, RNA-directed RNA polymerase beta chain, (2S)-1-[3-{[(2R)-2-hydroxypropyl]oxy}-2,2-bis({[(2R)-2-hydroxypropyl]oxy}methyl)propoxy]propan-2-ol, ... (4 entities in total)
• 機能のキーワード: rdrp, host-factor complex, translation, transferase
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm: P0CE48
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 279836.80

構造登録者

Kidmose, R.T.,Vasiliev, N.N.,Chetverin, A.B.,Knudsen, C.R.,Andersen, G.R. (登録日: 2010-04-20, 公開日: 2010-06-09, 最終更新日: 2023-09-06)

主引用文献

Kidmose, R.T.,Vasiliev, N.N.,Chetverin, A.B.,Andersen, G.R.,Knudsen, C.R.
Structure of the Qbeta replicase, an RNA-dependent RNA polymerase consisting of viral and host proteins.
Proc.Natl.Acad.Sci.USA, 107:10884-10889, 2010

PubMed Abstract: The RNA-dependent RNA polymerase core complex formed upon infection of Escherichia coli by the bacteriophage Qbeta is composed of the viral catalytic beta-subunit as well as the host translation elongation factors EF-Tu and EF-Ts, which are required for initiation of RNA replication. We have determined the crystal structure of the complex between the beta-subunit and the two host proteins to 2.5-A resolution. Whereas the basic catalytic machinery in the viral subunit appears similar to other RNA-dependent RNA polymerases, a unique C-terminal region of the beta-subunit engages in extensive interactions with EF-Tu and may contribute to the separation of the transient duplex formed between the template and the nascent product to allow exponential amplification of the phage genome. The evolution of resistance by the host appears to be impaired because of the interactions of the beta-subunit with parts of EF-Tu essential in recognition of aminoacyl-tRNA.

PubMed: 20534494
DOI: 10.1073/pnas.1003015107

実験手法

X-RAY DIFFRACTION (2.5 Å)