3MLW

Crystal structure of anti-HIV-1 V3 Fab 1006-15D in complex with an MN V3 peptide

3MLW の概要

• エントリーDOI: 10.2210/pdb3mlw/pdb
• 関連するPDBエントリー: 3MLR 3MLS 3MLT 3MLU 3MLV 3MLX 3MLY 3MLZ
• 分子名称: Human monoclonal anti-HIV-1 gp120 V3 antibody 1006-15D Fab light chain, Human monoclonal anti-HIV-1 gp120 V3 antibody 1006-15D Fab heavy chain, HIV-1 gp120 third variable region (V3) crown, ... (5 entities in total)
• 機能のキーワード: human monoclonal antibody, fab, hiv-1, gp120, third variable loop, antibody-antigen interaction, immune system
• 由来する生物種: Homo sapiens; 詳細
• 細胞内の位置: Surface protein gp120: Virion membrane ; Peripheral membrane protein . Transmembrane protein gp41: Virion membrane ; Single-pass type I membrane protein : P05877
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 101211.91

構造登録者

Kong, X.-P. (登録日: 2010-04-18, 公開日: 2010-07-14, 最終更新日: 2024-11-20)

主引用文献

Jiang, X.,Burke, V.,Totrov, M.,Williams, C.,Cardozo, T.,Gorny, M.K.,Zolla-Pazner, S.,Kong, X.P.
Conserved structural elements in the V3 crown of HIV-1 gp120.
Nat.Struct.Mol.Biol., 17:955-961, 2010

PubMed Abstract: Binding of the third variable region (V3) of the HIV-1 envelope glycoprotein gp120 to the cell-surface coreceptors CCR5 or CXCR4 during viral entry suggests that there are conserved structural elements in this sequence-variable region. These conserved elements could serve as epitopes to be targeted by a vaccine against HIV-1. Here we perform a systematic structural analysis of representative human anti-V3 monoclonal antibodies in complex with V3 peptides, revealing that the crown of V3 has four conserved structural elements: an arch, a band, a hydrophobic core and the peptide backbone. These are either unaffected by or are subject to minimal sequence variation. As these regions are targeted by cross-clade neutralizing human antibodies, they provide a blueprint for the design of vaccine immunogens that could elicit broadly cross-reactive protective antibodies.

PubMed: 20622876
DOI: 10.1038/nsmb.1861

実験手法

X-RAY DIFFRACTION (2.7 Å)