3MIT

Structure of Banana lectin-alpha-D-mannose complex

3MIT の概要

• エントリーDOI: 10.2210/pdb3mit/pdb
• 関連するPDBエントリー: 1x1v 3MIU 3MIV
• 分子名称: Lectin, alpha-D-mannopyranose, ZINC ION, ... (5 entities in total)
• 機能のキーワード: all beta sheet protein, beta prism-i fold, mannose specific, sugar binding protein
• 由来する生物種: Musa acuminata (Banana)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31169.61

構造登録者

Sharma, A.,Vijayan, M. (登録日: 2010-04-12, 公開日: 2010-09-08, 最終更新日: 2023-11-01)

主引用文献

Sharma, A.,Vijayan, M.
Influence of glycosidic linkage on the nature of carbohydrate binding in beta-prism I fold lectins: an X-ray and molecular dynamics investigation on banana lectin-carbohydrate complexes
Glycobiology, 21:23-33, 2011

PubMed Abstract: The three crystal structures reported here provide details of the interactions of mannose and the mannosyl-α-1,3-mannose component of a pentamannose with banana lectin and evidence for the binding of glucosyl-α-1,2-glucose to the lectin. The known structures involving the lectin include a complex with glucosyl-β-1,3-glucose. Modeling studies on the three disaccharide complexes with the reducing end and the nonreducing end at the primary binding site are also provided here. The results of the X-ray and modeling studies show that the disaccharides with an α-1,3 linkage prefer to have the nonreducing end at the primary binding site, whereas the reducing end is preferred at the site when the linkage is β-1,3 in mannose/glucose-specific β-prism I fold lectins. In the corresponding galactose-specific lectins, however, α-1,3-linked disaccharides cannot bind the lectin with the nonreducing end at the primary binding site on account of steric clashes with an aromatic residue that occurs only when the lectin is galactose-specific. Molecular dynamics simulations based on the known structures involving banana lectin enrich the information on lectin-carbohydrate interactions obtained from crystal structures. They demonstrate that conformational selection as well as induced fit operate when carbohydrates bind to banana lectin.

PubMed: 20729346
DOI: 10.1093/glycob/cwq128

実験手法

X-RAY DIFFRACTION (2.32 Å)