3MG3

Crystal structure of the orange carotenoid protein R155L mutant from cyanobacteria synechocystis sp. PCC 6803

「3I1X」から置き換えられました

3MG3 の概要

• エントリーDOI: 10.2210/pdb3mg3/pdb
• 関連するPDBエントリー: 1M98 3MG1 3MG2
• 分子名称: Orange carotenoid-binding protein, beta,beta-caroten-4-one, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: carotenoid binding protein, echinone, phycobilisome
• 由来する生物種: Synechocystis sp.
• 細胞内の位置: Cellular thylakoid membrane ; Peripheral membrane protein ; Cytoplasmic side : P74102
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72569.12

構造登録者

Wilson, A.,Kinney, J.,Zwart, P.H.,Punginelli, C.,D'Haen, S.,Perreau, F.,Klein, M.G.,Kirilovsky, D.,Kerfeld, C.A. (登録日: 2010-04-05, 公開日: 2010-04-14, 最終更新日: 2023-09-06)

主引用文献

Wilson, A.,Kinney, J.N.,Zwart, P.H.,Punginelli, C.,D'Haene, S.,Perreau, F.,Klein, M.G.,Kirilovsky, D.,Kerfeld, C.A.
Structural determinants underlying photoprotection in the photoactive orange carotenoid protein of cyanobacteria.
J.Biol.Chem., 285:18364-18375, 2010

PubMed Abstract: The photoprotective processes of photosynthetic organisms involve the dissipation of excess absorbed light energy as heat. Photoprotection in cyanobacteria is mechanistically distinct from that in plants; it involves the orange carotenoid protein (OCP), a water-soluble protein containing a single carotenoid. The OCP is a new member of the family of blue light-photoactive proteins; blue-green light triggers the OCP-mediated photoprotective response. Here we report structural and functional characterization of the wild type and two mutant forms of the OCP, from the model organism Synechocystis PCC6803. The structural analysis provides high resolution detail of the carotenoid-protein interactions that underlie the optical properties of the OCP, unique among carotenoid-proteins in binding a single pigment per polypeptide chain. Collectively, these data implicate several key amino acids in the function of the OCP and reveal that the photoconversion and photoprotective responses of the OCP to blue-green light can be decoupled.

PubMed: 20368334
DOI: 10.1074/jbc.M110.115709

実験手法

X-RAY DIFFRACTION (1.702 Å)