3MFK

Ets1 complex with stromelysin-1 promoter DNA

3MFK の概要

• エントリーDOI: 10.2210/pdb3mfk/pdb
• 分子名称: Protein C-ets-1, stromelysin-1 promoter DNA, ... (4 entities in total)
• 機能のキーワード: ets1, ets domain, protein-dna complex, stromelysin-1, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P14921
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47259.71

構造登録者

Babayeva, N.D.,Mino, K.,Tahirov, T.H. (登録日: 2010-04-02, 公開日: 2010-08-18, 最終更新日: 2023-09-06)

主引用文献

Babayeva, N.D.,Wilder, P.J.,Shiina, M.,Mino, K.,Desler, M.,Ogata, K.,Rizzino, A.,Tahirov, T.H.
Structural basis of Ets1 cooperative binding to palindromic sequences on stromelysin-1 promoter DNA.
Cell Cycle, 9:3054-3062, 2010

PubMed Abstract: Ets1 is a member of the Ets family of transcription factors. Ets1 is autoinhibited and its activation requires heterodimerization with a partner protein or DNA-mediated homodimerization for cooperative DNA binding. In the latter case, Ets1 molecules bind to palindromic sequences in which two Ets-binding sites (EBS) are separated by four base pairs, for example in the promoters of stromelysin-1 and p53. Interestingly, counteraction of autoinhibition requires the autoinhibitory region encoded by exon VII of the gene. The structural basis for the requirement of autoinhibitory sequences for Ets1 binding to palindromic EBS still remains unresolved. Here we report the crystal structure of two Ets1 molecules bound to an EBS palindrome of the stromelysin-1 promoter DNA, providing a plausible explanation for the requirement of exon VII-encoded sequences for Ets1 cooperative DNA binding. The proposed mechanism was verified both in vitro by surface plasmon resonance and in vivo by transcription-based assays.

PubMed: 20686355

実験手法

X-RAY DIFFRACTION (3 Å)