3MD0

Crystal structure of arginine/ornithine transport system ATPase from Mycobacterium tuberculosis bound to GDP (a RAS-like GTPase superfamily protein)

3MD0 の概要

• エントリーDOI: 10.2210/pdb3md0/pdb
• 関連するPDBエントリー: 3nxs 3p32 3tk1 4gt1
• 分子名称: Arginine/ornithine transport system ATPase, GUANOSINE-5'-DIPHOSPHATE, UNKNOWN ATOM OR ION, ... (4 entities in total)
• 機能のキーワード: seattle structural genomics center for infectious disease, ras, ras-like gtpase, allosteric modulator, tuberculosis, atp-binding, nucleotide-binding, ssgcid, transport protein
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39040.14

構造登録者

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2010-03-29, 公開日: 2010-04-21, 最終更新日: 2023-09-06)

主引用文献

Edwards, T.E.,Baugh, L.,Bullen, J.,Baydo, R.O.,Witte, P.,Thompkins, K.,Phan, I.Q.,Abendroth, J.,Clifton, M.C.,Sankaran, B.,Van Voorhis, W.C.,Myler, P.J.,Staker, B.L.,Grundner, C.,Lorimer, D.D.
Crystal structures of Mycobacterial MeaB and MMAA-like GTPases.
J.Struct.Funct.Genom., 16:91-99, 2015

PubMed Abstract: The methylmalonyl Co-A mutase-associated GTPase MeaB from Methylobacterium extorquens is involved in glyoxylate regulation and required for growth. In humans, mutations in the homolog methylmalonic aciduria associated protein (MMAA) cause methylmalonic aciduria, which is often fatal. The central role of MeaB from bacteria to humans suggests that MeaB is also important in other, pathogenic bacteria such as Mycobacterium tuberculosis. However, the identity of the mycobacterial MeaB homolog is presently unclear. Here, we identify the M. tuberculosis protein Rv1496 and its homologs in M. smegmatis and M. thermoresistibile as MeaB. The crystal structures of all three homologs are highly similar to MeaB and MMAA structures and reveal a characteristic three-domain homodimer with GDP bound in the G domain active site. A structure of Rv1496 obtained from a crystal grown in the presence of GTP exhibited electron density for GDP, suggesting GTPase activity. These structures identify the mycobacterial MeaB and provide a structural framework for therapeutic targeting of M. tuberculosis MeaB.

PubMed: 25832174
DOI: 10.1007/s10969-015-9197-2

実験手法

X-RAY DIFFRACTION (2.45 Å)