3MCD

Crystal structure of Helicobacter pylori MinE, a cell division topological specificity factor

3MCD の概要

• エントリーDOI: 10.2210/pdb3mcd/pdb
• 関連するPDBエントリー: 3KU7
• 分子名称: Cell division topological specificity factor (1 entity in total)
• 機能のキーワード: cytokinesis, mine, topological specificity factor, cell cycle, cell division
• 由来する生物種: Helicobacter pylori (Campylobacter pylori)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18431.34

構造登録者

Kang, G.B.,Song, H.E.,Kim, M.K.,Youn, H.S.,Lee, J.G.,An, J.Y.,Jeon, H.,Chun, J.S.,Eom, S.H. (登録日: 2010-03-29, 公開日: 2010-05-05, 最終更新日: 2024-03-20)

主引用文献

Kang, G.B.,Song, H.E.,Kim, M.K.,Youn, H.S.,Lee, J.G.,An, J.Y.,Chun, J.S.,Jeon, H.,Eom, S.H.
Crystal structure of Helicobacter pylori MinE, a cell division topological specificity factor
Mol.Microbiol., 76:1222-1231, 2010

PubMed Abstract: In Gram-negative bacteria, proper placement of the FtsZ ring, mediated by nucleoid occlusion and the activities of the dynamic oscillating Min proteins MinC, MinD and MinE, is required for correct positioning of the cell division septum. MinE is a topological specificity factor that counters the activity of MinCD division inhibitor at the mid-cell division site. Its structure consists of an anti-MinCD domain and a topology specificity domain (TSD). Previous NMR analysis of truncated Escherichia coli MinE showed that the TSD domain contains a long alpha-helix and two anti-parallel beta-strands, which mediate formation of a homodimeric alpha/beta structure. Here we report the crystal structure of full-length Helicobacter pylori MinE and redefine its TSD based on that structure. The N-terminal region of the TSD (residues 19-26), previously defined as part of the anti-MinCD domain, forms a beta-strand (betaA) and participates in TSD folding. In addition, H. pylori MinE forms a dimer through the interaction of anti-parallel betaA-strands. Moreover, we observed serial dimer-dimer interactions within the crystal packing, resulting in the formation of a multimeric structure. We therefore redefine the functional domain of MinE and propose that a multimeric filamentous structure is formed through anti-parallel beta-strand interactions.

PubMed: 20398219
DOI: 10.1111/j.1365-2958.2010.07160.x

実験手法

X-RAY DIFFRACTION (3.2 Å)