3MC8

POTRA1-3 of the periplasmic domain of Omp85 from Anabaena

3MC8 の概要

• エントリーDOI: 10.2210/pdb3mc8/pdb
• 関連するPDBエントリー: 3MC9
• 分子名称: Alr2269 protein (2 entities in total)
• 機能のキーワード: polypeptide transport associated, potra, outer bacterial membrane, protein membrane transport, beta barrel biogenesis, membrane protein
• 由来する生物種: Nostoc sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34722.55

構造登録者

Koenig, P.,Schleiff, E.,Sinning, I.,Tews, I. (登録日: 2010-03-28, 公開日: 2010-04-21, 最終更新日: 2024-04-03)

主引用文献

Koenig, P.,Mirus, O.,Haarmann, R.,Sommer, M.S.,Sinning, I.,Schleiff, E.,Tews, I.
Conserved properties of polypeptide transport-associated (POTRA) domains derived from cyanobacterial Omp85.
J.Biol.Chem., 285:18016-18024, 2010

PubMed Abstract: Proteins of the Omp85 family are conserved in all kingdoms of life. They mediate protein transport across or protein insertion into membranes and reside in the outer membranes of Gram-negative bacteria, mitochondria, and chloroplasts. Omp85 proteins contain a C-terminal transmembrane beta-barrel and a soluble N terminus with a varying number of polypeptide-transport-associated or POTRA domains. Here we investigate Omp85 from the cyanobacterium Anabaena sp. PCC 7120. The crystallographic three-dimensional structure of the N-terminal region shows three POTRA domains, here named P1 to P3 from the N terminus. Molecular dynamics simulations revealed a hinge between P1 and P2 but in contrast show that P2 and P3 are fixed in orientation. The P2-P3 arrangement is identical as seen for the POTRA domains from proteobacterial FhaC, suggesting this orientation is a conserved feature. Furthermore, we define interfaces for protein-protein interaction in P1 and P2. P3 possesses an extended loop unique to cyanobacteria and plantae, which influences pore properties as shown by deletion. It now becomes clear how variations in structure of individual POTRA domains, as well as the different number of POTRA domains with both rigid and flexible connections make the N termini of Omp85 proteins versatile adaptors for a plentitude of functions.

PubMed: 20348103
DOI: 10.1074/jbc.M110.112649

実験手法

X-RAY DIFFRACTION (2.59 Å)