3MBY

Ternary complex of DNA Polymerase BETA with template base A and 8oxodGTP in the active site with a dideoxy terminated primer

3MBY の概要

• エントリーDOI: 10.2210/pdb3mby/pdb
• 分子名称: DNA polymerase beta, DNA (5'-D(*CP*CP*GP*AP*CP*AP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3'), DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*(DOC))-3'), ... (9 entities in total)
• 機能のキーワード: nucleotidyl transferase, dna polymerase, a:(syn)8oxodgtp, a:c transversion, transferase, lyase-dna complex, lyase/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P06746
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 48398.68

構造登録者

Batra, V.K.,Beard, W.A.,Hou, E.W.,Pedersen, L.C.,Prasad, R.,Wilson, S.H. (登録日: 2010-03-26, 公開日: 2010-06-09, 最終更新日: 2023-09-06)

主引用文献

Batra, V.K.,Beard, W.A.,Hou, E.W.,Pedersen, L.C.,Prasad, R.,Wilson, S.H.
Mutagenic conformation of 8-oxo-7,8-dihydro-2'-dGTP in the confines of a DNA polymerase active site.
Nat.Struct.Mol.Biol., 17:889-890, 2010

PubMed Abstract: The major product of oxidative base damage is 8-oxo-7,8-dihydro-2'-deoxyguanine (8odG). The coding potential of this lesion is modulated by its glycosidic torsion angle that controls whether its Watson-Crick or Hoogsteen edge is used for base pairing. The 2.0-A structure of DNA polymerase (pol) beta bound with 8odGTP opposite template adenine indicates that the modified nucleotide assumes the mutagenic syn conformation and that the nonmutagenic anti conformation would be incompatible with efficient DNA synthesis.

PubMed: 20526335
DOI: 10.1038/nsmb.1852

実験手法

X-RAY DIFFRACTION (2 Å)