3MBK

The 1.35 A Structure of the Phosphatase Domain of the Suppressor of T Cell Receptor Signalling Protein in Complex with Sulphate

3MBK の概要

• エントリーDOI: 10.2210/pdb3mbk/pdb
• 関連するPDBエントリー: 2H0Q
• 分子名称: Ubiquitin-associated and SH3 domain-containing protein B, SULFATE ION (3 entities in total)
• 機能のキーワード: pgm, sts-1, signaling protein, low ph, nucleus, phosphoprotein, sh3 domain
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Cytoplasm : Q8BGG7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59582.25

構造登録者

Nassar, N.,Jakoncic, J. (登録日: 2010-03-25, 公開日: 2010-06-23, 最終更新日: 2023-09-06)

主引用文献

Jakoncic, J.,Sondgeroth, B.,Carpino, N.,Nassar, N.
The 1.35 A resolution structure of the phosphatase domain of the suppressor of T-cell receptor signaling protein in complex with sulfate.
Acta Crystallogr.,Sect.F, 66:643-647, 2010

PubMed Abstract: The suppressor of T-cell signaling (Sts) proteins are multidomain proteins that negatively regulate the signaling of membrane-bound receptors, including the T-cell receptor (TCR) and the epidermal growth-factor receptor (EGFR). They contain at their C-terminus a 2H-phosphatase homology (PGM) domain that is responsible for their protein tyrosine phosphatase activity. Here, the crystal structure of the phosphatase domain of Sts-1, Sts-1(PGM), was determined at pH 4.6. The asymmetric unit contains two independent molecules and each active site is occupied by a sulfate ion. Each sulfate is located at the phosphate-binding site and makes similar interactions with the catalytic residues. The structure suggests an explanation for the lower Michaelis-Menten constants at acidic pH.

PubMed: 20516590
DOI: 10.1107/S1744309110014259

実験手法

X-RAY DIFFRACTION (1.35 Å)