3MAW

Structure of the Newcastle disease virus F protein in the post-fusion conformation

3MAW の概要

• エントリーDOI: 10.2210/pdb3maw/pdb
• 分子名称: Fusion glycoprotein F0, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-alpha-D-glucopyranose (3 entities in total)
• 機能のキーワード: fusion protein, class i, paramyxovirus, glycoprotein, viral protein
• 由来する生物種: Newcastle disease virus (NDV)
• 細胞内の位置: Virion membrane; Single-pass type I membrane protein (By similarity): P12572
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 104469.72

構造登録者

Jardetzky, T.S.,Wen, X. (登録日: 2010-03-24, 公開日: 2010-05-26, 最終更新日: 2024-11-06)

主引用文献

Swanson, K.,Wen, X.,Leser, G.P.,Paterson, R.G.,Lamb, R.A.,Jardetzky, T.S.
Structure of the Newcastle disease virus F protein in the post-fusion conformation.
Virology, 402:372-379, 2010

PubMed Abstract: The paramyxovirus F protein is a class I viral membrane fusion protein which undergoes a significant refolding transition during virus entry. Previous studies of the Newcastle disease virus, human parainfluenza virus 3 and parainfluenza virus 5 F proteins revealed differences in the pre- and post-fusion structures. The NDV Queensland (Q) F structure lacked structural elements observed in the other two structures, which are key to the refolding and fusogenic activity of F. Here we present the NDV Australia-Victoria (AV) F protein post-fusion structure and provide EM evidence for its folding to a pre-fusion form. The NDV AV F structure contains heptad repeat elements missing in the previous NDV Q F structure, forming a post-fusion six-helix bundle (6HB) similar to the post-fusion hPIV3 F structure. Electrostatic and temperature factor analysis of the F structures points to regions of these proteins that may be functionally important in their membrane fusion activity.

PubMed: 20439109
DOI: 10.1016/j.virol.2010.03.050

実験手法

X-RAY DIFFRACTION (3.5 Å)