3M76

Crystal Structure of Plant SLAC1 homolog TehA

3M76 の概要

• エントリーDOI: 10.2210/pdb3m76/pdb
• 関連するPDBエントリー: 3M71 3M72 3M73 3M74 3M75 3M77 3M78 3M7B 3M7C 3M7E 3M7L
• 分子名称: Tellurite resistance protein tehA homolog, octyl beta-D-glucopyranoside (3 entities in total)
• 機能のキーワード: anion channel, alpha helical integral membrane protein, structural genomics, psi-2, protein structure initiative, new york consortium on membrane protein structure, nycomps, plant slac-1 homolog, unknown function
• 由来する生物種: Haemophilus influenzae
• 細胞内の位置: Cell inner membrane ; Multi- pass membrane protein : P44741
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36480.06

構造登録者

Chen, Y.-H.,Hendrickson, W.A.,New York Consortium on Membrane Protein Structure (NYCOMPS) (登録日: 2010-03-16, 公開日: 2010-05-12, 最終更新日: 2024-02-21)

主引用文献

Chen, Y.H.,Hu, L.,Punta, M.,Bruni, R.,Hillerich, B.,Kloss, B.,Rost, B.,Love, J.,Siegelbaum, S.A.,Hendrickson, W.A.
Homologue structure of the SLAC1 anion channel for closing stomata in leaves.
Nature, 467:1074-1080, 2010

PubMed Abstract: The plant SLAC1 anion channel controls turgor pressure in the aperture-defining guard cells of plant stomata, thereby regulating the exchange of water vapour and photosynthetic gases in response to environmental signals such as drought or high levels of carbon dioxide. Here we determine the crystal structure of a bacterial homologue (Haemophilus influenzae) of SLAC1 at 1.20 Å resolution, and use structure-inspired mutagenesis to analyse the conductance properties of SLAC1 channels. SLAC1 is a symmetrical trimer composed from quasi-symmetrical subunits, each having ten transmembrane helices arranged from helical hairpin pairs to form a central five-helix transmembrane pore that is gated by an extremely conserved phenylalanine residue. Conformational features indicate a mechanism for control of gating by kinase activation, and electrostatic features of the pore coupled with electrophysiological characteristics indicate that selectivity among different anions is largely a function of the energetic cost of ion dehydration.

PubMed: 20981093
DOI: 10.1038/nature09487

実験手法

X-RAY DIFFRACTION (1.5 Å)