3M4W

Structural basis for the negative regulation of bacterial stress response by RseB

3M4W の概要

• エントリーDOI: 10.2210/pdb3m4w/pdb
• 関連するPDBエントリー: 2P4B
• 分子名称: Sigma-E factor regulatory protein rseB, Sigma-E factor negative regulatory protein, ZINC ION, ... (4 entities in total)
• 機能のキーワード: rsea, rseb, rsep, stress response, sigma factor, periplasm, cell membrane, transmembrane, signaling protein-signaling protein complex, signaling protein/signaling protein
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Periplasm (Potential): P0AFX9; Cell membrane; Single-pass membrane protein (Potential): P0AFX7
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 176582.30

構造登録者

Kim, D.Y.,Kwon, E.,Choi, J.K.,Hwang, H.-Y.,Kim, K.K. (登録日: 2010-03-12, 公開日: 2010-05-05, 最終更新日: 2023-11-01)

主引用文献

Kim, D.Y.,Kwon, E.,Choi, J.K.,Hwang, H.-Y.,Kim, K.K.
Structural basis for the negative regulation of bacterial stress response by RseB
Protein Sci., 19:1258-1263, 2010

PubMed Abstract: The sigmaE-dependent stress response in bacterial cells is initiated by the DegS- and RseP-regulated intramembrane proteolysis of a membrane-spanning antisigma factor, RseA. RseB binds to RseA and inhibits its sequential cleavage, thereby functioning as a negative modulator of this response. In the crystal structure of the periplasmic domain of RseA bound to RseB, the DegS cleavage site of RseA is unstructured, however, its P1 residue is buried in the hydrophobic pocket of RseB, which suggests that RseB binding blocks the access of DegS to the cleavage site.

PubMed: 20512978
DOI: 10.1002/pro.393

実験手法

X-RAY DIFFRACTION (2.3 Å)