3M3F
PEPA bound to the ligand binding domain of GluA3 (flop form)
Summary for 3M3F
| Entry DOI | 10.2210/pdb3m3f/pdb |
| Related | 3M3K 3M3L |
| Descriptor | Glutamate receptor 3, GLUTAMIC ACID, 2-[2,6-difluoro-4-({2-[(phenylsulfonyl)amino]ethyl}sulfanyl)phenoxy]acetamide, ... (5 entities in total) |
| Functional Keywords | glutamate receptor, glua3, glur3, ampa receptor, neurotransmitter receptor, s1s2, transport protein |
| Biological source | Rattus norvegicus (brown rat,rat,rats) More |
| Cellular location | Cell membrane ; Multi-pass membrane protein : P19492 |
| Total number of polymer chains | 1 |
| Total formula weight | 29650.78 |
| Authors | Ahmed, A.H.,Ptak, C.P.,Oswald, R.E. (deposition date: 2010-03-09, release date: 2010-03-23, Last modification date: 2024-11-20) |
| Primary citation | Ahmed, A.H.,Ptak, C.P.,Oswald, R.E. Molecular mechanism of flop selectivity and subsite recognition for an AMPA receptor allosteric modulator: structures of GluA2 and GluA3 in complexes with PEPA. Biochemistry, 49:2843-2850, 2010 Cited by PubMed Abstract: Glutamate receptors are important potential drug targets for cognitive enhancement and the treatment of schizophrenia in part because they are the most prevalent excitatory neurotransmitter receptors in the vertebrate central nervous system. One approach to the application of therapeutic agents to the AMPA subtype of glutamate receptors is the use of allosteric modulators, which promote dimerization by binding to a dimer interface thereby reducing the degree of desensitization and deactivation. AMPA receptors exist in two alternatively spliced variants (flip and flop) that differ in desensitization and receptor activation profiles. Most of the structural information about modulators of the AMPA receptor targets the flip subtype. We report here the crystal structure of the flop-selective allosteric modulator, PEPA, bound to the binding domains of the GluA2 and GluA3 flop isoforms of AMPA receptors. Specific hydrogen bonding patterns can explain the preference for the flop isoform. This includes a bidentate hydrogen bonding pattern between PEPA and N754 of the flop isoforms of GluA2 and GluA3 (the corresponding position in the flip isoform is S754). Comparison with other allosteric modulators provides a framework for the development of new allosteric modulators with preferences for either the flip or flop isoforms. In addition to interactions with N/S754, specific interactions of the sulfonamide with conserved residues in the binding site are characteristics of a number of allosteric modulators. These, in combination with variable interactions with five subsites on the binding surface, lead to different stoichiometries, orientations within the binding pockets, and functional outcomes. PubMed: 20199107DOI: 10.1021/bi1000678 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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