3M29

Crystallographic and Single Crystal Spectral Analysis of the Peroxidase Ferryl Intermediate

Summary for 3M29

• Entry DOI: 10.2210/pdb3m29/pdb
• Related: 1dsg 1dso 1zby 2v23 3M23 3M25 3M26 3M27 3M28 3M2A 3M2B 3M2C 3M2D 3M2E 3M2F 3M2G 3M2H 3M2I 3e2n 3e2o 5CCP
• Descriptor: Cytochrome c peroxidase, mitochondrial, PROTOPORPHYRIN IX CONTAINING FE, PHOSPHATE ION, ... (4 entities in total)
• Functional Keywords: cytochrome c peroxidase (ccp), oxidoreductase, heme, hydrogen peroxide, iron, metal-binding, mitochondrion, organic radical, peroxidase
• Biological source: Saccharomyces cerevisiae (yeast)
• Cellular location: Mitochondrion matrix: P00431
• Total number of polymer chains: 1
• Total formula weight: 34026.47

Authors

Meharenna, Y.T.,Poulos, T.L. (deposition date: 2010-03-06, release date: 2010-05-12, Last modification date: 2024-02-21)

Primary citation

Meharenna, Y.T.,Doukov, T.,Li, H.,Soltis, S.M.,Poulos, T.L.
Crystallographic and single-crystal spectral analysis of the peroxidase ferryl intermediate.
Biochemistry, 49:2984-2986, 2010

PubMed Abstract: The ferryl [Fe(IV)O] intermediate is important in many heme enzymes, and thus, the precise nature of the Fe(IV)-O bond is critical in understanding enzymatic mechanisms. The 1.40 A crystal structure of cytochrome c peroxidase Compound I has been determined as a function of X-ray dose while the visible spectrum was being monitored. The Fe-O bond increases in length from 1.73 A in the low-X-ray dose structure to 1.90 A in the high-dose structure. The low-dose structure correlates well with an Fe(IV) horizontal lineO bond, while we postulate that the high-dose structure is the cryo-trapped Fe(III)-OH species previously thought to be an Fe(IV)-OH species.

PubMed: 20230048
DOI: 10.1021/bi100238r

Experimental method

X-RAY DIFFRACTION (1.4 Å)