3LX1

Crystal Structure analysis of PCNA1 from Thermococcus kodakaraensis tk0535

3LX1 の概要

• エントリーDOI: 10.2210/pdb3lx1/pdb
• 関連するPDBエントリー: 3LX2
• 分子名称: DNA polymerase sliding clamp 1, SULFATE ION (3 entities in total)
• 機能のキーワード: pcna, dna processivity factor, trimer, toroidal, dna replication, dna-binding, dna binding protein
• 由来する生物種: Thermococcus kodakarensis (Thermococcus kodakaraensis)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29387.50

構造登録者

Ladner, J.E.,Kelman, Z.,Pan, M. (登録日: 2010-02-24, 公開日: 2011-01-26, 最終更新日: 2024-02-21)

主引用文献

Ladner, J.E.,Pan, M.,Hurwitz, J.,Kelman, Z.
Crystal structures of two active proliferating cell nuclear antigens (PCNAs) encoded by Thermococcus kodakaraensis.
Proc.Natl.Acad.Sci.USA, 108:2711-2716, 2011

PubMed Abstract: Proliferating cell nuclear antigen (PCNA) is a ring-shaped protein that encircles duplex DNA and plays an essential role in many DNA metabolic processes in archaea and eukarya. The eukaryotic and euryarchaea genomes contain a single gene encoding for PCNA. Interestingly, the genome of the euryarchaeon Thermococcus kodakaraensis contains two PCNA-encoding genes (TK0535 and TK0582), making it unique among the euryarchaea kingdom. It is shown here that the two T. kodakaraensis PCNA proteins support processive DNA synthesis by the polymerase. Both proteins form trimeric structures with characteristics similar to those of other archaeal and eukaryal PCNA proteins. One of the notable differences between the TK0535 and TK0582 rings is that the interfaces are different, resulting in different stabilities for the two trimers. The possible implications of these observations for PCNA functions are discussed.

PubMed: 21270332
DOI: 10.1073/pnas.1019179108

実験手法

X-RAY DIFFRACTION (2 Å)