3LVH

Crystal structure of a clathrin heavy chain and clathrin light chain complex

3LVH の概要

• エントリーDOI: 10.2210/pdb3lvh/pdb
• 関連するPDBエントリー: 3LVG
• 分子名称: Clathrin heavy chain 1, Clathrin light chain B (2 entities in total)
• 機能のキーワード: self assembly, coated pit, cytoplasmic vesicle, membrane, calcium, structural protein
• 由来する生物種: Bos taurus (bovine,cow,domestic cattle,domestic cow); 詳細
• 細胞内の位置: Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side: P49951
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 281053.57

構造登録者

Wilbur, J.D.,Hwang, P.K.,Ybe, J.A.,Lane, M.,Sellers, B.D.,Jacobson, M.P.,Fletterick, R.J.,Brodsky, F.M. (登録日: 2010-02-20, 公開日: 2010-06-09, 最終更新日: 2024-02-21)

主引用文献

Wilbur, J.D.,Hwang, P.K.,Ybe, J.A.,Lane, M.,Sellers, B.D.,Jacobson, M.P.,Fletterick, R.J.,Brodsky, F.M.
Conformation switching of clathrin light chain regulates clathrin lattice assembly.
Dev.Cell, 18:841-848, 2010

PubMed Abstract: Clathrin-coated vesicle formation is responsible for membrane traffic to and from the endocytic pathway during receptor-mediated endocytosis and organelle biogenesis, influencing how cells relate to their environment. Generating these vesicles involves self-assembly of clathrin molecules into a latticed coat on membranes that recruits receptors and organizes protein machinery necessary for budding. Here we define a molecular mechanism regulating clathrin lattice formation by obtaining structural information from co-crystals of clathrin subunits. Low resolution X-ray diffraction data (7.9-9.0 A) was analyzed using a combination of molecular replacement with an energy-minimized model and noncrystallographic symmetry averaging. Resulting topological information revealed two conformations of the regulatory clathrin light chain bound to clathrin heavy chain. Based on protein domain positions, mutagenesis, and biochemical assays, we identify an electrostatic interaction between the clathrin subunits that allows the observed conformational variation in clathrin light chains to alter the conformation of the clathrin heavy chain and thereby regulates assembly.

PubMed: 20493816
DOI: 10.1016/j.devcel.2010.04.007

実験手法

X-RAY DIFFRACTION (9 Å)